The effects of aB-crystallin on mitochondrial death pathway during
hydrogen peroxide induced apoptosis

Deng, Gonghua; Li, Junli; Liu, Meidong; Liu, Shuang; Tu, Zizhi; Xiao, Xianzhong

The effects of aB-crystallin on mitochondrial death pathway during hydrogen peroxide induced apoptosis

dc.contributor.author	Deng, Gonghua	de
dc.contributor.author	Li, Junli	de
dc.contributor.author	Liu, Meidong	de
dc.contributor.author	Liu, Shuang	de
dc.contributor.author	Tu, Zizhi	de
dc.contributor.author	Xiao, Xianzhong	de
dc.date.accessioned	2008-06-17T13:57:02Z
dc.date.available	2008-06-17T13:57:02Z
dc.date.issued	2005-06-20	de
dc.description.abstract	aB-crystallin, a major small heat shock protein, has recently been shown to exert inhibitory effects on apoptosis, while the responsible mechanisms remain largely unknown. In the present study, we discovered that aB-crystallin protected mouse myoblast C2C12 cells against oxidative stress-induced apoptosis. During hydrogen peroxide-induced apoptosis, aBcrystallin showed that it decreased the redistribution level of phosphatidylserine (PS), reduced the release of cytochrome C and Smac/Diablo from mitochondria into cytoplasm, and decreased the cleavage of Bid. Interestingly, immunoprecipitation experiments with anti-aBcrystallin and anti-myc-tag antibodies demonstrated respectively an interaction between aBcrystallin and p53 during hydrogen peroxide induced apoptosis. Both the NH2-terminal and COOH-terminal regions of aB-crystallin could interact with p53, suggesting two domains of aB-crystallin are necessary for the interaction. Electrophoresis mobility shift assay (EMSA) and luciferase assay further demonstrated that aB-crystallin inhibited the upregulation of the DNA-binding, as well as the transactivation activity of p53 induced by hydrogen peroxide. Our results show that aB-crystallin has a protective role in oxidative stress induced apoptosis by interference with the mitochondrial death pathway.	en
dc.identifier.issn	1611-2156	de
dc.identifier.uri	http://hdl.handle.net/2003/25647
dc.identifier.uri	http://dx.doi.org/10.17877/DE290R-8177
dc.language.iso	en	de
dc.relation.ispartofseries	EXCLI Journal ; Vol. 4, 2005	en
dc.subject	aB-crystallin	en
dc.subject	apoptosis	en
dc.subject	Bid	en
dc.subject	Cytochrome C	en
dc.subject	hydrogen peroxide	en
dc.subject	mitochondria	en
dc.subject	p53	en
dc.subject	Smac/Diablo	en
dc.subject.ddc	610
dc.title	The effects of aB-crystallin on mitochondrial death pathway during hydrogen peroxide induced apoptosis	en
dc.type	Text	de
dc.type.publicationtype	article	de
dcterms.accessRights	open access
eldorado.dnb.zdberstkatid	2132560-1

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