Myxococcus xanthus as host for the production of benzoxazoles

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dc.contributor.authorWinand, Lea
dc.contributor.authorLernoud, Lucia
dc.contributor.authorMeyners, Saskia Anna
dc.contributor.authorKuhr, Katharina
dc.contributor.authorHiller, Wolf
dc.contributor.authorNett, Markus
dc.date.accessioned2023-10-18T13:28:54Z
dc.date.available2023-10-18T13:28:54Z
dc.date.issued2022-12-09
dc.description.abstractBenzoxazoles are important structural motifs in pharmaceutical drugs. Here, we present the heterologous production of 3-hydroxyanthranilate-derived benzoxazoles in the host bacterium Myxococcus xanthus following the expression of two genes from the nataxazole biosynthetic gene cluster of Streptomyces sp. Tü 6176. The M. xanthus expression strain achieved a benzoxazole titer of 114.6±7.4 mg L−1 upon precursor supplementation, which is superior to other bacterial production systems. Crosstalk between the heterologously expressed benzoxazole pathway and the endogenous myxochelin pathway led to the combinatorial biosynthesis of benzoxazoles featuring a 2,3-dihydroxybenzoic acid (2,3-DHBA) building block. Subsequent in vitro studies confirmed that this crosstalk is not only due to the availability of 2,3-DHBA in M. xanthus, rather, it is promoted by the adenylating enzyme MxcE from the myxochelin pathway, which contributes to the activation of aryl carboxylic acids and delivers them to benzoxazole biosynthesis.en
dc.identifier.urihttp://hdl.handle.net/2003/42159
dc.identifier.urihttp://dx.doi.org/10.17877/DE290R-23992
dc.language.isoende
dc.relation.ispartofseriesChemBioChem;24(5)
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/de
dc.subjectBenzoxazoleen
dc.subjectCombinatorial biosynthesisen
dc.subjectHeterologous expressionen
dc.subjectLigasesen
dc.subjectMyxococcus xanthusla
dc.subject.ddc660
dc.subject.rswkBenzoxazolde
dc.subject.rswkKombinatorische Synthesede
dc.subject.rswkBiosynthesede
dc.subject.rswkHeterologe Genexpressionde
dc.subject.rswkLigasende
dc.subject.rswkMyxococcus xanthusde
dc.titleMyxococcus xanthus as host for the production of benzoxazolesen
dc.typeTextde
dc.type.publicationtypeResearchArticlede
dcterms.accessRightsopen access
eldorado.secondarypublicationtruede
eldorado.secondarypublication.primarycitationWinand, L., Lernoud, L., Meyners, S. A., Kuhr, K., Hiller, W., Nett, M., ChemBioChem 2023, 24, e202200635.de
eldorado.secondarypublication.primaryidentifierDOI: https://doi.org/10.1002/cbic.202200635de

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