Cellular Characterization of SARS Coronavirus Nucleocapsid

Choi, Yook-Wah; Fielding, Burtram; Goh, Phuay-Yee; Hong, Wanjin; Lim, Seng Gee; Ooi, Eng-Eong; Shuo, Shen; Tan, Timothy H. P.; Tan, Yee-Joo

Cellular Characterization of SARS Coronavirus Nucleocapsid

dc.contributor.author	Choi, Yook-Wah	de
dc.contributor.author	Fielding, Burtram	de
dc.contributor.author	Goh, Phuay-Yee	de
dc.contributor.author	Hong, Wanjin	de
dc.contributor.author	Lim, Seng Gee	de
dc.contributor.author	Ooi, Eng-Eong	de
dc.contributor.author	Shuo, Shen	de
dc.contributor.author	Tan, Timothy H. P.	de
dc.contributor.author	Tan, Yee-Joo	de
dc.date.accessioned	2008-06-17T13:46:51Z
dc.date.available	2008-06-17T13:46:51Z
dc.date.issued	2004-11-01	de
dc.description.abstract	The Severe and Acute Respiratory Syndrome coronavirus (SARS CoV) is a newly-emerged virus that caused an outbreak of atypical pneumonia in the winter of 2002-2003. Polyclonal antibodies raised against the nucleocapsid (N) of the SARS CoV showed the localization of N to the cytoplasm and the nucleolus in virus-infected and N-expressing Vero E6 cells. Like other coronavirus N proteins, the SARS N is probably a phosphoprotein. N protein expressed in mammalian cells is apparently able to "spread" to neighboring cells. For N to spread to neighboring cells, it must be exported out of the expressing cells. This is shown by the immunoprecipitation of N from the culture medium of a stable cell line expressing myc-N. Deletion studies showed that the 27 kD C-terminal domain of N (C1/2) is the minimal region of N that can spread to other cells. The nucleolar localization and spreading of N are artefacts of fixation, reminiscent of other protein-transduction domain (PTD)-containing proteins.	en
dc.identifier.issn	1611-2156	de
dc.identifier.uri	http://hdl.handle.net/2003/25640
dc.identifier.uri	http://dx.doi.org/10.17877/DE290R-14553
dc.language.iso	en	de
dc.relation.ispartofseries	EXCLI Journal ; Vol. 3, 2004	en
dc.subject	coronavirus	en
dc.subject	fixation	en
dc.subject	nucleocapsid	en
dc.subject	nucleolus	en
dc.subject	SARS	en
dc.subject	transduction	en
dc.subject.ddc	610
dc.title	Cellular Characterization of SARS Coronavirus Nucleocapsid	en
dc.type	Text	de
dc.type.publicationtype	article	de
dcterms.accessRights	open access
eldorado.dnb.zdberstkatid	2132560-1

Files

Original bundle

Now showing 1 - 1 of 1

Name:: Goh10-04proofrev.pdf
Size:: 541.27 KB
Format:: Adobe Portable Document Format
Description:: DNB

Download

Collections

Original Articles