The effects of ovarian cancer cell-derived exosomes on vascular endothelial growth factor expression in endothelial cells
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Date
2019-10-09
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Abstract
Ovarian carcinoma is considered as a major clinical challenge worldwide. Exosomes, nano-sized
intraluminal vesicles of multivesicular bodies, are secreted by most types of cells and play an
important role in intercellular communication. Cancer cell-derived exosomes can develop cancer
progression and metastasis by manipulating the local and distant biological environments.
Angiogenesis is an important contributor to tumor progression. Vascular endothelial growth factor
(VEGF) is the most potent pro-angiogenic protein and induces proliferation, sprouting, and vessel
formation by endothelial cells. In this study, exosomes derived from ovarian epithelial cancer
cells OVACAR-3 (exo-OVCAR-3) were successfully isolated and characterized by scanning electron
microscopy in terms of size and morphology. Cellular internalization of exosomes labeled with PKH
fluorescent dye was monitored by a fluorescence microscope. Our results elucidated that exosomes
treatment (100 µg/ml) had a promoting effect on VEGF expression and secretion in endothelial cells.
Furthermore, we demonstrated that exo-OVCAR-3 caused an increase in the proliferation and migration
rate of endothelial cells. It seems that inducing VEGF by
exo-OVCAR-3 can influence the vascular behavior of endothelial cells in vitro.
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Keywords
Ovarian cancer, Cancer cell-derived exosomes, Exo-OVCAR-3, Vascular endothelial growth factor