Escherichia coli K88 Interaction with IgA Oligosaccharides

Acedo-Felix, Evelia; Ramos-Clamont M, Gabriela; Vázquez-Moreno, Luz; Winzerling, Joy

Escherichia coli K88 Interaction with IgA Oligosaccharides

dc.contributor.author	Acedo-Felix, Evelia	de
dc.contributor.author	Ramos-Clamont M, Gabriela	de
dc.contributor.author	Vázquez-Moreno, Luz	de
dc.contributor.author	Winzerling, Joy	de
dc.date.accessioned	2008-06-17T14:13:37Z
dc.date.available	2008-06-17T14:13:37Z
dc.date.issued	2007-01-26	de
dc.description.abstract	Diarrhea from enterotoxigenic Escherichia coli (E. coli) expressing the K88 fimbrial adhesin causes high morbidity and mortality among newborn and weaned piglets. K88 fimbrial adhesins are surface filaments with lectin activity that recognize specific glycoconjugates (glycoproteins or glycolipids) on the surface of intestinal cells. Carbohydrates that compete for adhesion attachment could serve as an alternative for disease prevention. In this study, IgA, IgG and IgM oligosaccharides were tested to inhibit the adhesion of E. coli K88 to piglet mucins. Immunoglobulins were isolated from porcine serum by hydrophobic interaction chromatography (HIC) and purified by affinity chromatography. In vitro K88 adhesin interacts specifically with IgA oligosaccharides, but not with carbohydrates of IgG or IgM. Also IgA oligosaccharides partially inhibit the adherence of K88 strain to porcine intestinal mucins.	en
dc.identifier.issn	1611-2156	de
dc.identifier.uri	http://hdl.handle.net/2003/25679
dc.identifier.uri	http://dx.doi.org/10.17877/DE290R-8289
dc.language.iso	en	de
dc.relation.ispartofseries	EXCLI Journal ; Vol. 6, 2007	en
dc.subject	affinity purification	en
dc.subject	E. coli K88 adhesin	en
dc.subject	IgA oligosaccharides	en
dc.subject	inhibition of adhesion	en
dc.subject	piglet mucins	en
dc.subject.ddc	610
dc.title	Escherichia coli K88 Interaction with IgA Oligosaccharides	en
dc.type	Text	de
dc.type.publicationtype	article	de
dcterms.accessRights	open access
eldorado.dnb.zdberstkatid	2132560-1

Files

Original bundle

Now showing 1 - 1 of 1

Name:: Vazquez-Moreno21-06proof1.pdf
Size:: 282.58 KB
Format:: Adobe Portable Document Format
Description:: DNB

Download

Collections

Original Articles