Epigenetic chromatin modification by amber suppression technology
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Date
2018-02-22
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Abstract
The genetic incorporation of unnatural amino acids (UAAs) into proteins by amber suppression
technology provides unique avenues to study protein structure, function and interactions both in vitro
and in living cells and organisms. This approach has been particularly useful for studying mechanisms
of epigenetic chromatin regulation, since these extensively involve dynamic changes in structure,
complex formation and posttranslational modifications that are difficult to access by traditional
approaches. Here, we review recent achievements in this field, emphasizing UAAs that help to unravel
protein-protein interactions in cells by photo-crosslinking or that allow the biosynthesis of proteins
with defined posttranslational modifications for studying their function and turnover in vitro and in
cells.
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Keywords
Genetic Code Expansion, Unnatural Amino Acids, UV-crosslinking, Post-translational modifications, Bioorthogonal Chemistry