Epigenetic chromatin modification by amber suppression technology

Neumann, Heinz; Neumann-Staubitz, Petra; Witte, Anna; Summerer, Daniel

Epigenetic chromatin modification by amber suppression technology

dc.contributor.author	Neumann, Heinz
dc.contributor.author	Neumann-Staubitz, Petra
dc.contributor.author	Witte, Anna
dc.contributor.author	Summerer, Daniel
dc.date.accessioned	2022-07-15T09:35:28Z
dc.date.available	2022-07-15T09:35:28Z
dc.date.issued	2018-02-22
dc.description.abstract	The genetic incorporation of unnatural amino acids (UAAs) into proteins by amber suppression technology provides unique avenues to study protein structure, function and interactions both in vitro and in living cells and organisms. This approach has been particularly useful for studying mechanisms of epigenetic chromatin regulation, since these extensively involve dynamic changes in structure, complex formation and posttranslational modifications that are difficult to access by traditional approaches. Here, we review recent achievements in this field, emphasizing UAAs that help to unravel protein-protein interactions in cells by photo-crosslinking or that allow the biosynthesis of proteins with defined posttranslational modifications for studying their function and turnover in vitro and in cells.	de
dc.identifier.uri	http://hdl.handle.net/2003/40994
dc.identifier.uri	http://dx.doi.org/10.17877/DE290R-22843
dc.language.iso	en	de
dc.subject	Genetic Code Expansion	de
dc.subject	Unnatural Amino Acids	de
dc.subject	UV-crosslinking	de
dc.subject	Post-translational modifications	de
dc.subject	Bioorthogonal Chemistry	de
dc.subject.ddc	570
dc.subject.ddc	540
dc.title	Epigenetic chromatin modification by amber suppression technology	de
dc.type	Text	de
dc.type.publicationtype	article	de
dcterms.accessRights	open access
eldorado.openaire.projectidentifier	info:eu-repo/grantAgreement/EC/H2020/723863/EU/Programmable Readers, Writers, and Erasers of the Epigenetic Cytosine Code/EPICODE	de
eldorado.secondarypublication	true	de
eldorado.secondarypublication.primarycitation	Current opinion in chemical biology. Volume 45, August 2018, Pages 1-9	de
eldorado.secondarypublication.primaryidentifier	https://doi.org/10.1016/j.cbpa.2018.01.017	de

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