Anti-inflammatory effect of selagin-7-O-(6''-O-acetyl-)-ß-D-glycoside isolated from Cancrinia discoidea on lipopolysaccharide-induced mouse macrophage RAW 264.7 cells
| dc.contributor.author | Xiao, Kai-Jun | |
| dc.contributor.author | Wang, Wen-Xia | |
| dc.contributor.author | Dai, Jia-Li | |
| dc.contributor.author | Zhu, Liang | |
| dc.date.accessioned | 2014-11-27T12:47:09Z | |
| dc.date.available | 2014-11-27T12:47:09Z | |
| dc.date.issued | 2014-09-09 | |
| dc.description.abstract | Selagin-7-O-(6''-O-acetyl-)-β-D-glycoside, a new flavone glycoside isolated from Cancrinia discoidea, is known to exhibit anti-inflammatory activity in vivo. This study aimed to investigate the protection of this flavone glycoside on inflammation in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. The effects of selagin-7-O-(6''-O-acetyl-)-β-D-glycoside on inflammatory cytokines and signaling pathways were analyzed by enzyme-linked immunosorbent assay, reverse transcription-polymerase chain reaction, and western blot. Results show that selagin-7-O-(6''-O-acetyl-)-β-D-glycoside protected LPS-induced macrophage RAW 264.7 cells from injury. The flavone glycoside markedly inhibited the LPS-induced production of tumor necrosis factor-α, interleukin-1β, and interleukin-6 and increased interleukin-10 release in a concentration-dependent manner. Furthermore, treatment with the flavone glycoside decreased nitric oxide and prostaglandin E2 in LPS-challenged RAW 264.7 cells. These decreases were associated with the down-regulation of inducible nitric oxide synthase (iNOS), cyclooxygenase (COX-2), and nuclear factor kappa B (NF-κB) activity. These findings suggest that the anti-inflammatory effects of selagin-7-O-(6''-O-acetyl-)-β-D-glycoside were associated with the adjustment of in flammatory cytokines, and attributed to the down-regulation of NF-κB and consequent suppression of the expression of iNOS and COX-2. | en |
| dc.identifier.issn | 1611-2156 | |
| dc.identifier.uri | http://hdl.handle.net/2003/33735 | |
| dc.identifier.uri | http://dx.doi.org/10.17877/DE290R-7043 | |
| dc.language.iso | en | |
| dc.relation.ispartofseries | EXCLI Journal ; Vol. 13, 2014 | en |
| dc.subject | selagin-7-O-(6''-O-acetyl-)- β -D-glycoside | en |
| dc.subject | LPS | en |
| dc.subject | macrophage | en |
| dc.subject | cytokine | en |
| dc.subject | Nuclear factor- κB | en |
| dc.subject.ddc | 610 | |
| dc.title | Anti-inflammatory effect of selagin-7-O-(6''-O-acetyl-)-ß-D-glycoside isolated from Cancrinia discoidea on lipopolysaccharide-induced mouse macrophage RAW 264.7 cells | en |
| dc.type | Text | |
| dc.type.publicationtype | article | |
| dcterms.accessRights | open access | |
| eldorado.dnb.zdberstkatid | 2132560-1 |