Biotechnological production of cyclic dinucleotides - challenges and opportunities

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dc.contributor.authorBartsch, Tabea
dc.contributor.authorBecker, Martin
dc.contributor.authorRolf, Jascha
dc.contributor.authorRosenthal, Katrin
dc.contributor.authorLütz, Stephan
dc.date.accessioned2022-03-04T12:58:01Z
dc.date.available2022-03-04T12:58:01Z
dc.date.issued2021-12-25
dc.description.abstractCyclic dinucleotides (CDNs) are widely used secondary signaling molecules in prokaryotic and eukaryotic cells. As strong agonists of the stimulator of interferon genes, they are of great interest for pharmaceutical applications. In particular, cyclic-GMP-AMP and related synthetic CDNs are promising candidates in preclinical work and even some in clinical phase 1 and 2 studies. The comparison of chemical and biocatalytic synthesis routes elucidated that biological CDN synthesis offers some advantages, such as shorter synthesis time, avoiding complex protective group chemistry, and the access to a new spectrum of CDNs. However, the synthesis of CDNs in preparative quantities is still a challenge, since the chemical synthesis of CDNs suffers from low yields and complex synthetic routes and the enzymatically catalyzed synthesis is limited by low product titers and process stability. We aim to review the latest discoveries and recent trends in chemical and biocatalytic synthesis of CDNs with a focus on the synthesis of a huge variety of CDN derivatives. We furthermore consider the most promising biotechnological processes for CDN production by evaluating key figures of the currently known processes.en
dc.identifier.urihttp://hdl.handle.net/2003/40760
dc.identifier.urihttp://dx.doi.org/10.17877/DE290R-22618
dc.language.isoende
dc.relation.ispartofseriesBiotechnology and bioengineering;119(3)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectc-di-GMPen
dc.subjectcGAMPen
dc.subjectcGASen
dc.subjectCyclic dinucleotide (CDN)en
dc.subjectDncVen
dc.subjectNucleotidyltransferaseen
dc.subject.ddc660
dc.titleBiotechnological production of cyclic dinucleotides - challenges and opportunitiesen
dc.typeTextde
dc.type.publicationtypearticlede
dcterms.accessRightsopen access
eldorado.secondarypublicationtruede
eldorado.secondarypublication.primarycitationBartsch, T., Becker, M., Rolf, J., Rosenthal, K., & Lütz, S. (2022). Biotechnological production of cyclic dinucleotides—Challenges and opportunities. Biotechnology and Bioengineering, 119, 677– 684. https://doi.org/10.1002/bit.28027de
eldorado.secondarypublication.primaryidentifierhttps://doi.org/10.1002/bit.28027de

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Arbeitsgruppe Bioverfahrenstechnik