Simultaneous determination of sarcosine and its related metabolites by gas chromatography-tandem mass spectrometry for prostate cancer diagnosis

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dc.contributor.authorYamkamon, Vichanan
dc.contributor.authorYee, Pyone Pyone
dc.contributor.authorYainoi, Sakda
dc.contributor.authorEiamphungporn, Warawan
dc.contributor.authorSuksrichavalit, Thummaruk
dc.date.accessioned2019-03-25T07:37:57Z
dc.date.available2019-03-25T07:37:57Z
dc.date.issued2018-10-16
dc.description.abstractShortly after sarcosine was delineated as a potential biomarker for prostate cancer in 2009, a variety of analytical methods for clinical application were developed. Moreover, higher uptake of glycine in the mitochondria also played a role in cancer proliferation. A major constraint in the accurate quantification of sarcosine was the interference of the two isomers, α-alanine and β-alanine, using chromatographic separation techniques. Accordingly, we aimed to develop an analytical method for determining sarcosine and its related metabolites (α- and β-alanine, glycine and creatinine) under the same conditions by gas chromatography-tandem mass spectrometry (GCMS/ MS). BSTFA + 1 % TMCS was used for silylation, and GC-MS/MS conditions were optimized for the target analytes. The unique transition ions of sarcosine, α- and β-alanine, glycine and creatinine set up in MRM acquisition were m/z 116 → 73, 190 → 147, 176 → 147, 176 → 147 and 100 → 73, respectively. This newly developed method was successfully validated to apply in clinical settings with low limits of detection (0.01 - 0.03 μg•mL-1), high correlations (R2 > 0.99), great accuracy (88 – 110 % recovery), and notable precision (RSD < 10 %). All TMS derivatives were > 80 % stable for up to 2 h after derivatization and analyzing during this period promises to achieve an accurate result. Monitoring the five-substance profile could enhance prospects for early diagnosis of prostate cancer.en
dc.identifier.issn1611-2156
dc.identifier.urihttp://hdl.handle.net/2003/37954
dc.identifier.urihttp://dx.doi.org/10.17877/DE290R-19939
dc.language.isoen
dc.relation.ispartofseriesEXCLI Journal;Vol. 17 2018
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectGas chromatography-tandem mass spectrometryen
dc.subjectSarcosineen
dc.subjectAlanineen
dc.subjectGlycineen
dc.subjectCreatinineen
dc.subjectMRMen
dc.subject.ddc610
dc.titleSimultaneous determination of sarcosine and its related metabolites by gas chromatography-tandem mass spectrometry for prostate cancer diagnosisen
dc.typeText
dc.type.publicationtypearticle
dcterms.accessRightsopen access
eldorado.dnb.zdberstkatid2132560-1
eldorado.secondarypublicationtrue

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