Differential engulfment of Staphylococcus aureus and Pseudomonas aeruginosa by monocyte-derived macrophages is associated with altered phagocyte biochemistry and morphology

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dc.contributor.authorEl Mohtadi, Mohamed
dc.contributor.authorPilkington, Lisa
dc.contributor.authorLiauw, Christopher M.
dc.contributor.authorAshworth, Jason J.
dc.contributor.authorDempsey-Hibbert, Nina
dc.contributor.authorBelboul, Amina
dc.contributor.authorWhitehead, Kathryn A.
dc.date.accessioned2020-12-15T09:47:26Z
dc.date.available2020-12-15T09:47:26Z
dc.date.issued2020-09-30
dc.description.abstractKnowledge of changes in macrophages following bacterial engulfment is limited. U937-derived macrophages were incubated with Staphylococcus aureus or Pseudomonas aeruginosa. Morphological and biochemical changes in macrophages following host-pathogen interactions were visualized using Scanning Electron Microscopy (SEM) and Fourier-Transform Infrared Spectroscopy (FTIR) respectively. Principal Component Analysis (PCA) was used to assess the variability in the FTIR spectra. Following host-pathogen interactions, survival of S. aureus was significantly lower than P. aeruginosa (P<0.05) and cellular morphology of macrophages was different after incubation with S. aureus compared to P. aeruginosa. Following incubation with S. aureus macrophages were more globular and amorphous in shape whereas long linear pseudopodia were observed following incubation with P. aeruginosa. Distinct FTIR spectra were identified in macrophages post interaction with the different bacteria and PCA analysis demonstrated distinct biochemical differences in the phagocytes following engulfment of the bacteria, with > 99 % of variability in the FTIR spectra explained by the first two principal components. These findings demonstrated that there were clear morphological and biochemical changes in macrophages following engulfment of two different bacterial types suggesting that the biochemical components of the bacterial cell wall influenced the biochemical characteristics and hence the morphology of macrophages in distinct ways.en
dc.identifier.citationEl Mohtadi, M., Pilkington, L., Liauw, C. M., Ashworth, J. J., Dempsey-Hibbert, N., Belboul, A., & Whitehead, K. A. (2020). Differential engulfment of Staphylococcus aureus and Pseudomonas aeruginosa by monocyte-derived macrophages is associated with altered phagocyte biochemistry and morphology. EXCLI Journal, 19, 1372-1384. https://doi.org/10.17179/excli2020-2766en
dc.identifier.issn1611-2156
dc.identifier.urihttp://hdl.handle.net/2003/39897
dc.identifier.urihttp://dx.doi.org/10.17877/DE290R-21787
dc.language.isoen
dc.publisherIfADo - Leibniz Research Centre for Working Environment and Human Factors, Dortmunden
dc.relation.ispartofseriesEXCLI Journal;Vol. 19 2020
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectMacrophagesen
dc.subjectBacteriaen
dc.subjectPhagocytosisen
dc.subjectFTIRen
dc.subjectCell membraneen
dc.subjectMorphologyen
dc.subject.ddc610
dc.titleDifferential engulfment of Staphylococcus aureus and Pseudomonas aeruginosa by monocyte-derived macrophages is associated with altered phagocyte biochemistry and morphologyen
dc.typeText
dc.type.publicationtypearticle
dcterms.accessRightsopen access
eldorado.dnb.zdberstkatid2132560-1
eldorado.identifier.urlhttps://www.excli.de/index.php/excli/article/view/2766
eldorado.secondarypublicationtrue

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