Variability of antioxidant and biological activities of Rhus tripartitum related to phenolic compounds

Simple item page
dc.contributor.authorBen Miled, Hanène
dc.contributor.authorSaada, Mariem
dc.contributor.authorJallali, Ines
dc.contributor.authorBen Barka, Zaineb
dc.contributor.authorTlili, Mounira
dc.contributor.authorAlimi, Hichem
dc.contributor.authorSakly, Mohsen
dc.contributor.authorBen Rhouma, Khémais
dc.contributor.authorAbderrabba, Manef
dc.contributor.authorAbdelmelek, Hafedh
dc.contributor.authorTebourbi, Olfa
dc.contributor.authorKsouri, Riadh
dc.date.accessioned2017-10-18T12:42:44Z
dc.date.available2017-10-18T12:42:44Z
dc.date.issued2017-03-31
dc.description.abstractRhus species are known in traditional medicine for their therapeutic virtue and their extracts showed numerous important properties including antimalarial, antimicrobial, antiviral, and hypoglycemic and anticonvulsant activi- ties. Rhus tripartitum (Ucria) is a medicinal plant widely used in Tunisia folk medicine against chronic diarrhea and gastric ulcer. This study was designed to examine in vitro and ex vivo antioxidant, anti-inflammatory and anticancer activities of four extracts of Rhus tripartitum root cortex with increasing solvent polarity (hexane, di-chloromethane, methanol and water). HPLC was used to identify and quantify phenolic compounds in Rhus ex- tract. Water extract showed the highest antioxidant activity using oxygen radical absorbance capacity (ORAC method) with 8.95 ± 0.47 µmol Trolox/mg and a cell based-assay with 0.28 ± 0.12 µmol Trolox/mg as compared to the other fractions. Moreover, methanol extract displayed the strongest anti-cancer activity against human lung carcinoma (A-549) and colon adenocarcinoma cell lines (DLD-1) with an IC50 value of 60.69 ± 2.58 and 39.83 ± 4.56 µg/ml (resazurin test) and 44.52 ± 5.96 and 55.65 ± 6.00 µg/ml (hoechst test), respectively. Besides, the highest anti-inflammatory activity, inhibiting nitric oxide (NO) release, was exhibited by dichloromethane extract with 31.5 % at 160 µg/ml in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. The HPLC analysis showed that catechol and kaempferol were the major phenolics. These data suggest the richness of all fractions of les with different polarity and confirm the known traditional therapeutics virtues of this species for the treatment of dysentery, diarrhea and gastric ulcer.en
dc.identifier.issn1611-2156
dc.identifier.urihttp://hdl.handle.net/2003/36122
dc.identifier.urihttp://dx.doi.org/10.17877/DE290R-18138
dc.language.isoen
dc.relation.ispartofseriesEXCLI Journal;Vol. 16 2017
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectRhus tripartitumen
dc.subjectphenolicsen
dc.subjectanticancer abilityen
dc.subjectantioxidant capacityen
dc.subjectanti-inflammatory activityen
dc.subject.ddc610
dc.titleVariability of antioxidant and biological activities of Rhus tripartitum related to phenolic compoundsde
dc.typeText
dc.type.publicationtypearticle
dcterms.accessRightsopen access
eldorado.dnb.zdberstkatid2132560-1
eldorado.secondarypublicationtrue

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Ben_Miled_31032017_proof.pdf
Size:
204.68 KB
Format:
Adobe Portable Document Format
Description:
DNB
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
4.85 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Original Articles