Application of the PAMONO-sensor for quantification of microvesicles and determination of nano-particle size distribution

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dc.contributor.authorShpacovitch, Victoria
dc.contributor.authorSidorenko, Irina
dc.contributor.authorLenssen, Jan Eric
dc.contributor.authorTemchura, Vladimir
dc.contributor.authorWeichert, Frank
dc.contributor.authorMüller, Heinrich
dc.contributor.authorÜberla, Klaus
dc.contributor.authorZybin, Alexander
dc.contributor.authorSchramm, Alexander
dc.contributor.authorHergenröder, Roland
dc.date.accessioned2020-01-03T11:18:12Z
dc.date.available2020-01-03T11:18:12Z
dc.date.issued2017-01-27
dc.description.abstractThe PAMONO-sensor (plasmon assisted microscopy of nano-objects) demonstrated an ability to detect and quantify individual viruses and virus-like particles. However, another group of biological vesicles—microvesicles (100–1000 nm)—also attracts growing interest as biomarkers of different pathologies and needs development of novel techniques for characterization. This work shows the applicability of a PAMONO-sensor for selective detection of microvesicles in aquatic samples. The sensor permits comparison of relative concentrations of microvesicles between samples. We also study a possibility of repeated use of a sensor chip after elution of the microvesicle capturing layer. Moreover, we improve the detection features of the PAMONO-sensor. The detection process utilizes novel machine learning techniques on the sensor image data to estimate particle size distributions of nano-particles in polydisperse samples. Altogether, our findings expand analytical features and the application field of the PAMONO-sensor. They can also serve for a maturation of diagnostic tools based on the PAMONO-sensor platform.en
dc.identifier.urihttp://hdl.handle.net/2003/38482
dc.identifier.urihttp://dx.doi.org/10.17877/DE290R-20401
dc.language.isoende
dc.relation.ispartofseriesSensors;17(2)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectPlasmonic sensorsen
dc.subjectSurface plasmon resonanceen
dc.subjectExtracellular vesiclesen
dc.subjectMicrovesiclesen
dc.subjectMachine learningen
dc.subjectDeep learningen
dc.subject.ddc660
dc.titleApplication of the PAMONO-sensor for quantification of microvesicles and determination of nano-particle size distributionen
dc.typeTextde
dc.type.publicationtypearticlede
dcterms.accessRightsopen access
eldorado.secondarypublicationtruede
eldorado.secondarypublication.primarycitationShpacovitch, V.; Sidorenko, I.; Lenssen, J.E.; Temchura, V.; Weichert, F.; Müller, H.; Überla, K.; Zybin, A.; Schramm, A.; Hergenröder, R. Application of the PAMONO-Sensor for Quantification of Microvesicles and Determination of Nano-Particle Size Distribution. Sensors 2017, 17, 244.de
eldorado.secondarypublication.primaryidentifierhttps://doi.org/10.3390/s17020244de

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