High yield functional expression, purification, and reconstitution of human sodium/D-glucose cotransporter 1 (hSGLT1) in Pichia pastoris & pilot studies for photoaffinity labeling of functional domains of SGLT1
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Universität Dortmund
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Cotransporter protein, Sodium/D-glucose cotransporter1 (SGLT1), Heterologous expression, Pichia pastoris, Escherichia coli, Messenger RNA, Ribonuclease E (RNase E) Reconstitution, Proteoliposomes, Alpha-methyl-D-glucopyranoside, D-glucose, Phlorizin, Arbutin, Photoaffinity labeling, 3-Azidophlorizin, Brush border membrane vesicles, MALDI mass spectrometry