High yield functional expression, purification, and reconstitution of human sodium/D-glucose cotransporter 1 (hSGLT1) in Pichia pastoris & pilot studies for photoaffinity labeling of functional domains of SGLT1

dc.contributor.advisor	Kinne, Rolf	de
dc.contributor.author	Tyagi, Navneet Kumar	de
dc.contributor.referee	Waldmann, H.	de
dc.date.accepted	2005
dc.date.accessioned	2005-05-31T10:57:48Z
dc.date.available	2005-05-31T10:57:48Z
dc.date.created	2005-05-20	de
dc.date.issued	2005-05-17	de
dc.format.extent	5255669 bytes
dc.format.extent	23040 bytes
dc.format.extent	6482944 bytes
dc.format.mimetype	application/pdf
dc.format.mimetype	application/msword
dc.format.mimetype	application/msword
dc.identifier.uri	http://hdl.handle.net/2003/21356
dc.identifier.uri	http://dx.doi.org/10.17877/DE290R-7860
dc.language.iso	en
dc.publisher	Universität Dortmund	de
dc.subject	Cotransporter protein	en
dc.subject	Sodium/D-glucose cotransporter1 (SGLT1)	en
dc.subject	Heterologous expression	en
dc.subject	Pichia pastoris	en
dc.subject	Escherichia coli	en
dc.subject	Messenger RNA	en
dc.subject	Ribonuclease E (RNase E) Reconstitution	en
dc.subject	Proteoliposomes	en
dc.subject	Alpha-methyl-D-glucopyranoside	en
dc.subject	D-glucose	en
dc.subject	Phlorizin	en
dc.subject	Arbutin	en
dc.subject	Photoaffinity labeling	en
dc.subject	3-Azidophlorizin	en
dc.subject	Brush border membrane vesicles	en
dc.subject	MALDI mass spectrometry	en
dc.subject.ddc	540	de
dc.title	High yield functional expression, purification, and reconstitution of human sodium/D-glucose cotransporter 1 (hSGLT1) in Pichia pastoris & pilot studies for photoaffinity labeling of functional domains of SGLT1	en
dc.type	Text	de
dc.type.publicationtype	doctoralThesis	de
dcterms.accessRights	open access

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