Epigenetic chromatin modification by amber suppression technology

dc.contributor.authorNeumann, Heinz
dc.contributor.authorNeumann-Staubitz, Petra
dc.contributor.authorWitte, Anna
dc.contributor.authorSummerer, Daniel
dc.date.accessioned2019-06-25T14:17:06Z
dc.date.available2019-06-25T14:17:06Z
dc.date.issued2018-08
dc.description.abstractThe genetic incorporation of unnatural amino acids (UAAs) into proteins by amber suppression technology provides unique avenues to study protein structure, function and interactions both in vitro and in living cells and organisms. This approach has been particularly useful for studying mechanisms of epigenetic chromatin regulation, since these extensively involve dynamic changes in structure, complex formation and posttranslational modifications that are difficult to access by traditional approaches. Here, we review recent achievements in this field, emphasizing UAAs that help to unravel protein-protein interactions in cells by photo-crosslinking or that allow the biosynthesis of proteins with defined posttranslational modifications for studying their function and turnover in vitro and in cells.de
dc.identifier.urihttp://hdl.handle.net/2003/38114
dc.identifier.urihttp://dx.doi.org/10.17877/DE290R-20095
dc.language.isoende
dc.subject.ddc570
dc.subject.ddc540
dc.titleEpigenetic chromatin modification by amber suppression technologyde
dc.typeTextde
dc.type.publicationtypereportde
dcterms.accessRightsopen access
eldorado.secondarypublicationtruede
eldorado.secondarypublication.primarycitationCurrent opinion in chemical biology. 2018, Aug;45:1-9de
eldorado.secondarypublication.primaryidentifierdoi: 10.1016/j.cbpa.2018.01.017.de

Files

Original bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
CurrOp_2018.pdf
Size:
4.03 MB
Format:
Adobe Portable Document Format
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
4.85 KB
Format:
Item-specific license agreed upon to submission
Description: