Molecular cloning and characterization of protein disulfide isomerase of Brugia malayi, a human lymphatic filarial parasite

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dc.contributor.authorVerma, Pravesh
dc.contributor.authorDoharey, Pawan Kumar
dc.contributor.authorYadav, Sunita
dc.contributor.authorOmer, Ankur
dc.contributor.authorSingh, Poonam
dc.contributor.authorSaxena, Jitendra Kumar
dc.date.accessioned2017-11-27T07:39:34Z
dc.date.available2017-11-27T07:39:34Z
dc.date.issued2017-06-01
dc.description.abstractLymphatic filariasis results in an altered lymphatic system and the abnormal enlargement of body parts, causing pain, serious disability and social stigma. Effective vaccines are still not available nowadays, drugs against the disease is required. Protein disulfide isomerase (PDI) is an essential catalyst of the endoplasmic reticulum which is involved in folding and chaperone activities in different biological systems. Here, we report the enzymatic characterization of a Brugia malayi Protein disulfide isomerase (BmPDI), which was expressed and purified from Escherichia coli BL21 (DE3). Western blotting analysis showed the recombinant BmPDI could be recognized by anti-BmPDI Rabbit serum. The rBmPDI exhibited an optimum activity at pH 8 and 40 °C. The enzyme was inhibited by aurin and PDI inhibitor. Recombinant BmPDI showed interaction with recombinant Brugia malayi calreticulin (rBmCRT). The three-dimensional model for BmPDI and BmCRT was generated by homology modelling. A total of 25 hydrogen bonds were found to be formed between two interfaces. There are 259 non-bonded contacts present in the BmPDI-BmCRT complex and 12 salt bridges were formed in the interaction.en
dc.identifier.issn1611-2156
dc.identifier.urihttp://hdl.handle.net/2003/36202
dc.identifier.urihttp://dx.doi.org/10.17877/DE290R-18216
dc.language.isoen
dc.relation.ispartofseriesEXCLI Journal;Vol. 16 2017
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectprotein disulfide isomeraseen
dc.subjectBrugia malayien
dc.subjectantifilarialsen
dc.subjecthomology modelling and dockingen
dc.subject.ddc610
dc.titleMolecular cloning and characterization of protein disulfide isomerase of Brugia malayi, a human lymphatic filarial parasiteen
dc.typeText
dc.type.publicationtypearticle
dcterms.accessRightsopen access
eldorado.dnb.zdberstkatid2132560-1
eldorado.secondarypublicationtrue

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