Enhanced ribozyme-catalyzed recombination and oligonucleotide assembly in peptide-RNA condensates

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dc.contributor.authorLe Vay, Kristian
dc.contributor.authorSong, Emilie Yeonwha
dc.contributor.authorGhosh, Basusree
dc.contributor.authorTang, T.-Y. Dora
dc.contributor.authorMutschler, Hannes
dc.date.accessioned2022-03-15T12:48:45Z
dc.date.available2022-03-15T12:48:45Z
dc.date.issued2021-09-27
dc.description.abstractThe ability of RNA to catalyze RNA ligation is critical to its central role in many prebiotic model scenarios, in particular the copying of information during self-replication. Prebiotically plausible ribozymes formed from short oligonucleotides can catalyze reversible RNA cleavage and ligation reactions, but harsh conditions or unusual scenarios are often required to promote folding and drive the reaction equilibrium towards ligation. Here, we demonstrate that ribozyme activity is greatly enhanced by charge-mediated phase separation with poly-L-lysine, which shifts the reaction equilibrium from cleavage in solution to ligation in peptide-RNA coaggregates and coacervates. This compartmentalization enables robust isothermal RNA assembly over a broad range of conditions, which can be leveraged to assemble long and complex RNAs from short fragments under mild conditions in the absence of exogenous activation chemistry, bridging the gap between pools of short oligomers and functional RNAs.en
dc.identifier.urihttp://hdl.handle.net/2003/40796
dc.identifier.urihttp://dx.doi.org/10.17877/DE290R-22653
dc.language.isoende
dc.relation.ispartofseriesAngew. Chem., Int. Ed. Engl.;60(50)
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/
dc.subjectCatalysisen
dc.subjectCoacervatesen
dc.subjectLigasesen
dc.subjectPeptidesen
dc.subjectRibozymesen
dc.subject.ddc570
dc.subject.ddc540
dc.titleEnhanced ribozyme-catalyzed recombination and oligonucleotide assembly in peptide-RNA condensatesen
dc.typeTextde
dc.type.publicationtypearticlede
dcterms.accessRightsopen access
eldorado.secondarypublicationtruede
eldorado.secondarypublication.primarycitationK. Le Vay, E. Y. Song, B. Ghosh, T.-Y. D. Tang, H. Mutschler, Angew. Chem. Int. Ed. 2021, 60, 26096.de
eldorado.secondarypublication.primaryidentifierhttps://doi.org/10.1002/anie.202109267de

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