Cytocompatibility of a conductive nanofibrous carbon nanotube/poly(L-lactic acid) composite scaffold intended for nerve tissue engineering

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dc.contributor.authorKabiri, Mahboubeh
dc.contributor.authorOraee-Yazdani, Saeed
dc.contributor.authorDodel, Masumeh
dc.contributor.authorHanaee-Ahvaz, Hana
dc.contributor.authorSoudi, Sara
dc.contributor.authorSeyedjafari, Ehsan
dc.contributor.authorSalehi, Mohammad
dc.contributor.authorSoleimani, Masoud
dc.date.accessioned2016-06-03T16:11:33Z
dc.date.available2016-06-03T16:11:33Z
dc.date.issued2015-07-27
dc.description.abstractThe purpose of this study was to fabricate a conductive aligned nanofibrous substrate and evaluate its suitability and cytocompatibility with neural cells for nerve tissue engineering purposes. In order to reach these goals, we first used electrospinning to fabricate single-walled carbon-nanotube (SWCNT) incorporated poly(L-Lactic acid) (PLLA) nanofibrous scaffolds and then assessed its cytocompatibility with olfactory ensheathing glial cells (OEC). The plasma treated scaffolds were characterized using scanning electron microscopy and water contact angle. OECs were isolated from olfactory bulb of GFP Sprague-Dawley rats and characterized using OEC specific markers via immunocytochemistry and flow cytometery. The cytocompatibility of the conductive aligned nano-featured scaffold was assessed using microscopy and MTT assay. We indicate that doping of PLLA polymer with SWCNT can augment the aligned nanosized substrate with conductivity, making it favorable for nerve tissue engineering. Our results demonstrated that SWCNT/PLLA composite scaffold promote the adhesion, growth, survival and proliferation of OEC. Regarding the ideal physical, topographical and electrical properties of the scaffold and the neurotrophic and migratory features of the OECs, we suggest this scaffold and the cell/scaffold construct as a promising platform for cell delivery to neural defects in nerve tissue engineering approaches.en
dc.identifier.doi10.17179/excli2015-282
dc.identifier.issn1611-2156
dc.identifier.urihttp://hdl.handle.net/2003/35021
dc.identifier.urihttp://dx.doi.org/10.17877/DE290R-17069
dc.language.isoen
dc.relation.ispartofseriesEXCLI Journal;Vol. 14, 2015
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectnerve tissue engineeringen
dc.subjectolfactory ensheathing cellsen
dc.subjectcarbon nanotubeen
dc.subjectcomposite scaffolden
dc.subjectelectrospun nanofiberen
dc.subject.ddc610
dc.titleCytocompatibility of a conductive nanofibrous carbon nanotube/poly(L-lactic acid) composite scaffold intended for nerve tissue engineeringen
dc.typeText
dc.type.publicationtypearticle
dcterms.accessRightsopen access
eldorado.dnb.zdberstkatid2132560-1

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