Engineered TALE repeats for enhanced imaging-based analysisof cellular 5-Methylcytosine
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Date
2020-09-20
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Abstract
Two‐TALE test: Engineered transcription‐activator‐like effector (TALE) repeats can be used to design programmable receptors for improved in situ imaging‐based analysis of 5 mC in user‐defined DNA sequences. Pairs of natural 5 mC‐discriminating and engineered 5 mC‐binding TALEs allow nucleotide resolution analysis of 5 mC with response of both TALEs. This provides an impulse for the design of programmable imaging probes for studying 5 mC functions in chromatin regulation with increased dynamic range.
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Keywords
DNA methylation, Epigenetics, Imaging probes, Membrane-less organelles