Periodic temperature changes drive the proliferation of self-replicating RNAs in vesicle populations

Simple item page
dc.contributor.authorSalibi, Elia
dc.contributor.authorPeter, Benedikt
dc.contributor.authorSchwille, Petra
dc.contributor.authorMutschler, Hannes
dc.date.accessioned2024-08-15T10:17:47Z
dc.date.available2024-08-15T10:17:47Z
dc.date.issued2023-03-03
dc.description.abstractGrowth and division of biological cells are based on the complex orchestration of spatiotemporally controlled reactions driven by highly evolved proteins. In contrast, it remains unknown how their primordial predecessors could achieve a stable inheritance of cytosolic components before the advent of translation. An attractive scenario assumes that periodic changes of environmental conditions acted as pacemakers for the proliferation of early protocells. Using catalytic RNA (ribozymes) as models for primitive biocatalytic molecules, we demonstrate that the repeated freezing and thawing of aqueous solutions enables the assembly of active ribozymes from inactive precursors encapsulated in separate lipid vesicle populations. Furthermore, we show that encapsulated ribozyme replicators can overcome freezing-induced content loss and successive dilution by freeze-thaw driven propagation in feedstock vesicles. Thus, cyclic freezing and melting of aqueous solvents – a plausible physicochemical driver likely present on early Earth – provides a simple scenario that uncouples compartment growth and division from RNA self-replication, while maintaining the propagation of these replicators inside new vesicle populations.en
dc.identifier.urihttp://hdl.handle.net/2003/42648
dc.identifier.urihttp://dx.doi.org/10.17877/DE290R-24485
dc.language.isoende
dc.relation.ispartofseriesNature communications;14
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/de
dc.subjectMembrane biophysicsen
dc.subjectChemical origin of lifeen
dc.subjectRibozymesen
dc.subjectRNAen
dc.subjectSynthetic biologyen
dc.subject.ddc570
dc.subject.ddc540
dc.titlePeriodic temperature changes drive the proliferation of self-replicating RNAs in vesicle populationsen
dc.typeTextde
dc.type.publicationtypeArticlede
dcterms.accessRightsopen access
eldorado.openaire.projectidentifierinfo:eu-repo/grantAgreement/EC/H2020/802000/EU/Resurrecting LUCA - Engineering of RNA-encoded Cellular Life Using Dual Evolution and Intergenomic Transplantation/RiboLife
eldorado.secondarypublicationtruede
eldorado.secondarypublication.primarycitationSalibi, E., Peter, B., Schwille, P. et al. Periodic temperature changes drive the proliferation of self-replicating RNAs in vesicle populations. Nat Commun 14, 1222 (2023). https://doi.org/10.1038/s41467-023-36940-zde
eldorado.secondarypublication.primaryidentifierhttps://doi.org/10.1038/s41467-023-36940-zde

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
s41467-023-36940-z.pdf
Size:
1.51 MB
Format:
Adobe Portable Document Format
Description:
DNB
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
4.85 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Chemische Biologie