6,7-Dimethoxy-4-methylcoumarin suppresses pro-inflammatory mediator expression through inactivation of the NF-kappaB and MAPK pathways in LPS-induced RAW 264.7 cells

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dc.contributor.authorKim, Kil-Nam
dc.contributor.authorYang, Hye-Won
dc.contributor.authorKo, Seok-Chun
dc.contributor.authorKo, Yeong-Jong
dc.contributor.authorKim, Eun-A
dc.contributor.authorRoh, Seong Woon
dc.contributor.authorKo, Eun-Yi
dc.contributor.authorAhn, Ginnae
dc.contributor.authorHeo, Soo-Jin
dc.contributor.authorJeon, You-Jin
dc.contributor.authorYoon, Weon-Jong
dc.contributor.authorHyun, Chang-Gu
dc.contributor.authorKim, Daekyung
dc.date.accessioned2014-11-25T13:19:49Z
dc.date.available2014-11-25T13:19:49Z
dc.date.issued2014-07-21
dc.description.abstractIn this study, we investigated the ability of 6,7-dimethoxy-4-methylcoumarin (DMC) to inhibit lipopolysaccharide (LPS)-induced expression of pro-inflammatory mediators in mouse macrophage (RAW 264.7) cells, and the molecular mechanism through which this inhibition occurred. Our results indicated that DMC down regulated LPS-induced nitric oxide (NO) synthase (iNOS) and cyclooxygenase-2 (COX-2) expression, thereby reducing the production of NO and prostaglandin E2 (PGE2) in LPS-activated RAW 264.7 cells. Furthermore, DMC suppressed LPS-induced production of pro-inflammatory cytokines such as interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α. To elucidate the mechanism underlying the anti- inflammatory activity of DMC, we assessed its effects on the mitogen-activated protein kinase (MAPK) pathway and the activity and expression of nuclear transcription factor kappa-B (NF-κB). The experiments demonstrated that DMC inhibited LPS-induced phosphorylation of extracellular signal-regulated kinases (ERKs), c-Jun N-terminal kinase (JNK), and p38. In addition, it attenuated LPS-induced NF-κB activation via the inhibition of IκB-α phosphorylation. Taken together, these data suggest that DMC exerts its anti-inflammatory effects in RAW 264.7 cells through the inhibition of LPS-stimulated NF-κB and MAPK signaling, thereby downregulating the expression of pro-inflammatory mediators.en
dc.identifier.issn1611-2156
dc.identifier.urihttp://hdl.handle.net/2003/33712
dc.identifier.urihttp://dx.doi.org/10.17877/DE290R-6945
dc.language.isoen
dc.relation.ispartofseriesEXCLI Journal ; Vol. 13, 2014en
dc.subject6,7-Dimethoxy-4-methylcoumarin (DMC)en
dc.subjectanti-inflammatoryen
dc.subjectRAW 264.7 cellsen
dc.subjectNF-κBen
dc.subjectMAPKsen
dc.subject.ddc610
dc.title6,7-Dimethoxy-4-methylcoumarin suppresses pro-inflammatory mediator expression through inactivation of the NF-kappaB and MAPK pathways in LPS-induced RAW 264.7 cellsen
dc.typeText
dc.type.publicationtypearticle
dcterms.accessRightsopen access
eldorado.dnb.zdberstkatid2132560-1

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