Anti-inflammatory effects of enzymatic hydrolysates of velvet antler in RAW 264.7 cells in vitro and zebrafish model

Simple item page
dc.contributor.authorLee, Seung-Hong
dc.contributor.authorYang, Hye-Won
dc.contributor.authorDing, Yuling
dc.contributor.authorWang, Yanmei
dc.contributor.authorJeon, You-Jin
dc.contributor.authorMoon, Sang-Ho
dc.contributor.authorJeon, Byong-Tae
dc.contributor.authorSung, Si-Heung
dc.date.accessioned2016-06-06T13:37:39Z
dc.date.available2016-06-06T13:37:39Z
dc.date.issued2015-10-20
dc.description.abstractEnzymatic hydrolysis has been successfully used for the extraction of numerous biologically active components from a wide variety of natural sources. In the present study, velvet antler was subjected to the extraction process using Alcalase protease. We analyzed bioactive components, such as uronic acid, sulfated-glycosaminoglycans (sulfated-GAGs), and sialic acid, present in the velvet antler Alcalase hydrolysate (VAAH) and assessed their anti-inflammatory effects in zebrafish as well as in vitro using cell lines. VAAH mainly contained uronic acid (78.22 mg/g) and sulfated-GAGs (50.47 mg/g), while the amount of sialic acid was negligible (5.55 mg/g). VAAH inhibited the production of nitric oxide (NO) by lipopolysaccharide (LPS)-induced cells in a dosedependent manner and the inhibitory effect of VAAH on NO production was higher than that of hot water extracts. VAAH treatment also reduced the expression of inflammatory mediators such as nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). Furthermore, we evaluated anti-inflammatory effects of VAAH using LPS-stimulated zebrafish. Treatment with LPS significantly increased cell death, NO, and reactive oxygen species (ROS) levels in zebrafish. Notably, VAAH significantly inhibited the extent of LPS-stimulated cell death and generation of NO and ROS in zebrafish. These results suggest that VAAH alleviated inflammation and cell death by inhibiting the generation of ROS induced by LPS treatment. Thus, VAAH could be used as a potential natural remedy with a trong anti-inflammatory effect. Taken together, we believe that based on our present results, enzymatic hydrolysis of velvet antler may be an effective process to make antler products acceptable as elements of health foods and nutraceutical components with increased biological activity.en
dc.identifier.doi10.17179/excli2015-481
dc.identifier.issn1611-2156
dc.identifier.urihttp://hdl.handle.net/2003/35055
dc.identifier.urihttp://dx.doi.org/10.17877/DE290R-17103
dc.language.isoen
dc.relation.ispartofseriesEXCLI Journal;Vol. 14, 2015en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectenzymatic hydrolysisen
dc.subjectvelvet antleren
dc.subjectbioactive componentsen
dc.subjectanti-inflammatory effecten
dc.subject.ddc610
dc.titleAnti-inflammatory effects of enzymatic hydrolysates of velvet antler in RAW 264.7 cells in vitro and zebrafish modelen
dc.typeText
dc.type.publicationtypearticle
dcterms.accessRightsopen access
eldorado.dnb.zdberstkatid2132560-1

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Sung_20102015_proof.pdf
Size:
784.58 KB
Format:
Adobe Portable Document Format
Description:
DNB
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
3.12 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Original Articles