Anti-inflammatory effect and mechanism of action of Lindera erythrocarpa essential oil in lipopolysaccharide-stimulated RAW264.7 cells
| dc.contributor.author | Ko, Yeong-Jong | |
| dc.contributor.author | Ahn, Ginnae | |
| dc.contributor.author | Ham, Young-Min | |
| dc.contributor.author | Song, Sang-Mock | |
| dc.contributor.author | Ko, Eun-Yi | |
| dc.contributor.author | Cho, Su-Hyeon | |
| dc.contributor.author | Yoon, Weon-Jong | |
| dc.contributor.author | Kim, Kil-Nam | |
| dc.date.accessioned | 2017-12-04T11:50:54Z | |
| dc.date.available | 2017-12-04T11:50:54Z | |
| dc.date.issued | 2017-08-29 | |
| dc.description.abstract | The aim of this study was to investigate the chemical constituents of Lindera erythrocarpa essential oil (LEO) by gas chromatography-mass spectrometry and evaluate their inhibitory effect on the expression of pro-inflammatory mediators in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. Fifteen compounds, accounting for 63.7 % of the composition of LEO, were identified. The main compounds were nerolidol (18.73 %), caryophyllene (14.41 %), α-humulene (7.73 %), germacrene-D (4.82 %), and α-pinene (4.47 %). LEO significantly inhibited the expression of inducible nitric oxide (NO) synthase and cyclooxygenase-2, and subsequent production of NO and prostaglandin E2. In addition, it reduced the release of pro-inflammatory cytokines in LPS-activated RAW264.7 cells. The molecular mechanism underlying the effect of LEO was associated with inhibition of the phosphorylation of mitogen-activated protein kinase (MAPK). Furthermore, LEO inhibited LPS-induced phosphorylation and degradation of inhibitor of kappa B-α, which is required for the activation of the p50 and p65 nuclear factor (NF)-κB subunits in RAW264.7 cells. Taken together, these data suggest that LEO exerted its anti-inflammatory effect by downregulating LPS-induced production of pro-inflammatory mediators through the inhibition of NF-κB and MAPK signaling in RAW264.7 cells. | en |
| dc.identifier.issn | 1611-2156 | |
| dc.identifier.uri | http://hdl.handle.net/2003/36229 | |
| dc.identifier.uri | http://dx.doi.org/10.17877/DE290R-18243 | |
| dc.language.iso | en | |
| dc.relation.ispartofseries | EXCLI Journal;Vol. 16 2017 | |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
| dc.subject | Lindera erythrocarpa | en |
| dc.subject | Anti-inflammatory | en |
| dc.subject | Essential oil | en |
| dc.subject | NF- κ B | en |
| dc.subject | MAPK | en |
| dc.subject.ddc | 610 | |
| dc.title | Anti-inflammatory effect and mechanism of action of Lindera erythrocarpa essential oil in lipopolysaccharide-stimulated RAW264.7 cells | en |
| dc.type | Text | |
| dc.type.publicationtype | article | |
| dcterms.accessRights | open access | |
| eldorado.dnb.zdberstkatid | 2132560-1 | |
| eldorado.secondarypublication | true |