Anti-inflammatory effect and mechanism of action of Lindera erythrocarpa essential oil in lipopolysaccharide-stimulated RAW264.7 cells

dc.contributor.authorKo, Yeong-Jong
dc.contributor.authorAhn, Ginnae
dc.contributor.authorHam, Young-Min
dc.contributor.authorSong, Sang-Mock
dc.contributor.authorKo, Eun-Yi
dc.contributor.authorCho, Su-Hyeon
dc.contributor.authorYoon, Weon-Jong
dc.contributor.authorKim, Kil-Nam
dc.date.accessioned2017-12-04T11:50:54Z
dc.date.available2017-12-04T11:50:54Z
dc.date.issued2017-08-29
dc.description.abstractThe aim of this study was to investigate the chemical constituents of Lindera erythrocarpa essential oil (LEO) by gas chromatography-mass spectrometry and evaluate their inhibitory effect on the expression of pro-inflammatory mediators in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. Fifteen compounds, accounting for 63.7 % of the composition of LEO, were identified. The main compounds were nerolidol (18.73 %), caryophyllene (14.41 %), α-humulene (7.73 %), germacrene-D (4.82 %), and α-pinene (4.47 %). LEO significantly inhibited the expression of inducible nitric oxide (NO) synthase and cyclooxygenase-2, and subsequent production of NO and prostaglandin E2. In addition, it reduced the release of pro-inflammatory cytokines in LPS-activated RAW264.7 cells. The molecular mechanism underlying the effect of LEO was associated with inhibition of the phosphorylation of mitogen-activated protein kinase (MAPK). Furthermore, LEO inhibited LPS-induced phosphorylation and degradation of inhibitor of kappa B-α, which is required for the activation of the p50 and p65 nuclear factor (NF)-κB subunits in RAW264.7 cells. Taken together, these data suggest that LEO exerted its anti-inflammatory effect by downregulating LPS-induced production of pro-inflammatory mediators through the inhibition of NF-κB and MAPK signaling in RAW264.7 cells.en
dc.identifier.issn1611-2156
dc.identifier.urihttp://hdl.handle.net/2003/36229
dc.identifier.urihttp://dx.doi.org/10.17877/DE290R-18243
dc.language.isoen
dc.relation.ispartofseriesEXCLI Journal;Vol. 16 2017
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectLindera erythrocarpaen
dc.subjectAnti-inflammatoryen
dc.subjectEssential oilen
dc.subjectNF- κ Ben
dc.subjectMAPKen
dc.subject.ddc610
dc.titleAnti-inflammatory effect and mechanism of action of Lindera erythrocarpa essential oil in lipopolysaccharide-stimulated RAW264.7 cellsen
dc.typeText
dc.type.publicationtypearticle
dcterms.accessRightsopen access
eldorado.dnb.zdberstkatid2132560-1
eldorado.secondarypublicationtrue

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