A-NGR fusion protein induces apoptosis in human cancer cells

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dc.contributor.authorMohammadi-Farsani, Azadeh
dc.contributor.authorHabibi-Roudkenar, Mehryar
dc.contributor.authorGolkar, Majid
dc.contributor.authorShokrgozar, Mohammad Ali
dc.contributor.authorJahanian-Najafabadi, Ali
dc.contributor.authorKhanAhmad, Hossein
dc.contributor.authorValiyari, Samira
dc.contributor.authorBouzari, Saeid
dc.date.accessioned2018-11-09T07:55:07Z
dc.date.available2018-11-09T07:55:07Z
dc.date.issued2018-06-25
dc.description.abstractThe NGR peptide is one of the well-known peptides for targeting tumor cells. It has the ability to target aminopeptidase N (CD13) on tumor cells or the tumor vascular endothelium. In this study, the NGR peptide was used for targeting A subunit of the Shiga toxin to cancer cells. The cytotoxic effect of the A-NGR fusion protein was assessed on HT1080, U937, HT29 cancer cells and MRC-5 normal cells. For this purpose, cells were treated with different concentrations of A-NGR (0.5-40 μg/ml). The evaluation of cell viability was achieved by MTT assay. Apoptosis was determined by annexin-V/PI double staining flow cytometry. Alterations in the mRNA expression of apoptosis - related genes were assessed by real time RT- PCR. The results showed that A-NGR fusion protein effectively inhibited the growth of HT1080 and U937 cancer cells in comparison to negative control (PBS) but for CD13-negative HT-29 cancer cells, only at high concentrations of fusion protein was inhibited growth recorded. On the other hand, A-NGR had little cytotoxic effect on MRC-5 normal cells. The flow cytometry results showed that A-NGR induces apoptosis. Furthermore, the results of real time RT-PCR revealed that A-NGR significantly increases the mRNA expression of caspase 3 and caspase 9. Conclusively, A-NGR fusion protein has the ability of targeting CD13-positive cancer cells, the cytotoxic effect on CD13-positive cancer cells as well as has low cytotoxic effect on normal cells.en
dc.identifier.issn1611-2156
dc.identifier.urihttp://hdl.handle.net/2003/37647
dc.identifier.urihttp://dx.doi.org/10.17877/DE290R-19642
dc.language.isoen
dc.relation.ispartofseriesEXCLI Journal;Vol. 17 2018
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectShiga toxinen
dc.subjectNGR peptideen
dc.subjectApoptosisen
dc.subjectCytotoxicityen
dc.subject.ddc610
dc.titleA-NGR fusion protein induces apoptosis in human cancer cellsen
dc.typeText
dc.type.publicationtypearticle
dcterms.accessRightsopen access
eldorado.dnb.zdberstkatid2132560-1
eldorado.secondarypublicationtrue

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