Dataset on nicotine-free, nontransgenic tobacco (Nicotiana tabacum l.) edited by CRISPR-Cas9

Schachtsiek, Julia; Stehle, Felix

Dataset on nicotine-free, nontransgenic tobacco (Nicotiana tabacum l.) edited by CRISPR-Cas9

dc.contributor.author	Schachtsiek, Julia
dc.contributor.author	Stehle, Felix
dc.date.accessioned	2019-10-04T11:03:04Z
dc.date.available	2019-10-04T11:03:04Z
dc.date.issued	2019-08-23
dc.description.abstract	This dataset in brief is related to the research letter entitled "Nicotine-free, nontransgenic tobacco (Nicotiana tabacuml.) edited by CRISPR-Cas9" [1]. Cured tobacco products with a significantly reduced nicotine content helps people to overcome their nicotine addiction. Here we summarize additional data and method descriptions of the generation process of a nicotine-free, nontransgenic tobacco plant. This included the cloning, transformation and regeneration of transgenic tobacco plants, followed by the analysis of the nicotine content and genomic modifications caused by CRISPR-Cas9 mediated gene editing. Subsequently, nicotine-free plants were screened for loss of T-DNA cassette, i.e. nontransgenity. Finally, a metabolic footprint was recorded by 1H NMR analysis.	de
dc.identifier.uri	http://hdl.handle.net/2003/38262
dc.identifier.uri	http://dx.doi.org/10.17877/DE290R-20232
dc.language.iso	en	de
dc.relation.ispartofseries	Data in Brief;26. 104395
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/
dc.subject	CRISPR-Cas9	de
dc.subject	Nicotine-free	de
dc.subject	Gene editing	de
dc.subject	Nontransgenic	de
dc.subject	Nicotiana tabacum	de
dc.subject	Non-addictive	de
dc.subject.ddc	660
dc.title	Dataset on nicotine-free, nontransgenic tobacco (Nicotiana tabacum l.) edited by CRISPR-Cas9	de
dc.type	Text	de
dc.type.publicationtype	article	de
dcterms.accessRights	open access
eldorado.secondarypublication	true	de
eldorado.secondarypublication.primarycitation	Data in Brief. 26. 2019, 104395	de
eldorado.secondarypublication.primaryidentifier	DOI: 10.1016/j.dib.2019.104395	de

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