Debottlenecking of an in vitro enzyme cascade using a combined model‐ and experiment‐based approach

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dc.contributor.authorSiedentop, Regine
dc.contributor.authorDziennus, Marlon
dc.contributor.authorLütz, Stephan
dc.contributor.authorRosenthal, Katrin
dc.date.accessioned2025-03-07T11:25:24Z
dc.date.available2025-03-07T11:25:24Z
dc.date.issued2023-02-10
dc.description.abstractDesigning a feasible in vitro enzyme cascade is a challenging task that is often preceded by optimization steps. Computational methods can increase the efficiency of the optimization process by providing a deeper insight into the system. In this study, bottlenecks of a farnesyl pyrophosphate-producing in vitro enzyme cascade were determined by a combination of in silico modeling and wet-lab experiments. ATP and the precursor-isomerizing enzyme isopentenyl diphosphate δ-isomerase were identified as limitations of the in vitro enzyme cascade.en
dc.identifier.urihttp://hdl.handle.net/2003/43520
dc.identifier.urihttp://dx.doi.org/10.17877/DE290R-25353
dc.language.isoen
dc.relation.ispartofseriesChemie - Ingenieur - Technik; 95(4)
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/
dc.subjectBiocatalysisen
dc.subjectEnzyme cascadesen
dc.subjectIn vitro biotransformationsen
dc.subjectKinetic modelsen
dc.subjectTerpenesen
dc.subject.ddc660
dc.titleDebottlenecking of an in vitro enzyme cascade using a combined model‐ and experiment‐based approachen
dc.typeText
dc.type.publicationtypeResearchArticle
dcterms.accessRightsopen access
eldorado.secondarypublicationtrue
eldorado.secondarypublication.primarycitationSiedentop, R., Dziennus, M., Lütz, S. and Rosenthal, K. (2023), Debottlenecking of an In Vitro Enzyme Cascade Using a Combined Model- and Experiment-Based Approach . Chemie Ingenieur Technik, 95: 543-548. https://doi.org/10.1002/cite.202200170
eldorado.secondarypublication.primaryidentifierhttps://doi.org/10.1002/cite.202200170

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