Prosopis juliflora leave extracts induce cell death of MCF-7, HepG2, and LS-174T cancer cell lines

dc.contributor.authorElbehairi, Serag Eldin I.
dc.contributor.authorEzzat Ahmed, Ahmed
dc.contributor.authorAlshati, Ali A.
dc.contributor.authorAl-Kahtani, Mohammed A.
dc.contributor.authorAlfaifi, Mohammad Y.
dc.contributor.authorAlsyaad, Khalid M.
dc.contributor.authorAlalmie, Ali Yahya A.
dc.contributor.authorElimam Ahamed, Mohammed M.
dc.contributor.authorMoustafa, Mahmoud F.
dc.contributor.authorAlhag, Sadeq K.
dc.contributor.authorAl-Abd, Ahmed M.
dc.contributor.authorAbbas, Ahmed M.
dc.date.accessioned2020-12-14T14:58:50Z
dc.date.available2020-12-14T14:58:50Z
dc.date.issued2020-09-09
dc.description.abstractProsopis juliflora (P. juliflora) is a widespread phreatophytic tree, which belongs to the Fabaceae family. The goal of the present study is to investigate the potential anti-cancer effect of P. juliflora leave extracts and to identify its chemical composition. For this purpose, MCF-7 (breast), HepG2 (liver), and LS-174T (colorectal) cancer cell lines were cultivated and incubated with various concentrations of P. juliflora leave extracts, and its impact on cell viability, proliferation, and cell cycle stages was investigated. P. juliflora leave extracts induced concentration-dependent cytotoxicity against all tested cancer cell lines. The calculated IC50 was 18.17, 33.1 and 41.9 μg/ml for MCF-7, HePG2 and LS-174T, respectively. Detailed analysis revealed that the cytotoxic action of P. juliflora extracts was mainly via necrosis but not apoptosis. Moreover, DNA content flow cytometry analysis showed cell-specific anti-proliferative action and cell cycle stages arrest. In order to identify the anti-cancer constituents of P. juliflora, the ethyl extracts were analyzed by liquid chromatography-mass spectrometry. The major constituents identified in the ethyl extracts of P. juliflora leaves were hydroxymethyl-pyridine, nicotinamide, adenine, and poly-(methyl methacrylate) (PMMA). In conclusion, P. juliflora ethyl acetate extracts have a potential anti-cancer effect against breast adenocarcinoma, hepatocellular carcinoma, and colorectal adenocarcinoma, and is enriched with anti-cancer constituents.en
dc.identifier.citationElbehairi, S. E. I., Ezzat Ahmed, A., Alshati, A. A., Al-Kahtani, M. A., Alfaifi, M. Y., Alsyaad, K. M., Alalmie, A. Y. A. ., Elimam Ahamed, M. M., Moustafa, M. F., Alhag, S. K., Al-Abd, A. M., & Abbas, A. M. (2020). Prosopis juliflora leave extracts induce cell death of MCF-7, HepG2, and LS-174T cancer cell lines. EXCLI Journal, 19, 1282-1294. https://doi.org/10.17179/excli2020-2830de
dc.identifier.issn1611-2156
dc.identifier.urihttp://hdl.handle.net/2003/39892
dc.identifier.urihttp://dx.doi.org/10.17877/DE290R-21782
dc.language.isoen
dc.publisherIfADo - Leibniz Research Centre for Working Environment and Human Factors, Dortmundde
dc.relation.ispartofseriesEXCLI Journal;Vol. 19. 2020, pp.1282-1294
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectProsopis julifloraen
dc.subjectCytotoxicityen
dc.subjectApoptosisen
dc.subjectcanceren
dc.subjectMCF-7en
dc.subjectHepG2en
dc.subjectLS-174Ten
dc.subject.ddc610
dc.titleProsopis juliflora leave extracts induce cell death of MCF-7, HepG2, and LS-174T cancer cell linesen
dc.typeText
dc.type.publicationtypearticle
dcterms.accessRightsopen access
eldorado.dnb.zdberstkatid2132560-1
eldorado.identifier.urlhttps://www.excli.de/index.php/excli/article/view/2830
eldorado.secondarypublicationtrue

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