Tumor-derived urinary exosomal long non-coding RNAs as diagnostic biomarkers for bladder cancer

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dc.contributor.authorAbbastabar, Maryam
dc.contributor.authorSarfi, Mohammad
dc.contributor.authorGolestani, Abolfazl
dc.contributor.authorKarimi, Ali
dc.contributor.authorPourmand, Gholamreza
dc.contributor.authorKhalili, Ehsan
dc.date.accessioned2020-12-07T06:59:18Z
dc.date.available2020-12-07T06:59:18Z
dc.date.issued2020-03-04
dc.description.abstractBladder cancer (BC) is the sixth most common malignancy in men and 17th in women. Exosomal long non-coding RNAs (lncRNAs) have been defined as a novel biomarker for BC. The aim of this study is to evaluate the clinical significance of urine exosomal PVT-1, ANRIL and PCAT-1 as a biomarker in BC patients with tumors classified as T1 or T2. Exosomes were isolated from urine of BC patients and healthy donors, then characterized according to their shape, size, and exosome markers by Electron Microscopy, Dynamic light scattering, and Western blotting. Exosomal lncRNAs extraction was done to determine the expression levels of PVT-1, ANRIL and PCAT-1 by qRT-PCR. ANRIL and PCAT-1 expression was significantly higher in BC patients compared to normal subjects. To evaluate the performance of the identified lncRNAs for BC detection, we performed ROC curves analysis. The diagnostic accuracy of ANRIL and PCAT-1, measured by AUC, was 0.7229 (sensitivity = 46.67 % and specificity = 87.5 %) and 0.7292 (sensitivity = 43.33 % and specificity = 87.5 %). Transcript levels of lncRNAs in urinary exosomes are potential diagnostic biomarkers in bladder cancer.en
dc.identifier.citationhttps://doi.org/10.17179/excli2019-1683de
dc.identifier.issn1611-2156
dc.identifier.urihttp://hdl.handle.net/2003/39832
dc.identifier.urihttp://dx.doi.org/10.17877/DE290R-21723
dc.language.isoen
dc.publisherIfADo - Leibniz Research Centre for Working Environment and Human Factors, Dortmunden
dc.relation.ispartofseriesEXCLI Journal;Vol. 19 2020
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectLncRNAen
dc.subjectExosomeen
dc.subjectBladder canceren
dc.subjectUrineen
dc.subject.ddc610
dc.titleTumor-derived urinary exosomal long non-coding RNAs as diagnostic biomarkers for bladder canceren
dc.typeText
dc.type.publicationtypearticle
dcterms.accessRightsopen access
eldorado.dnb.zdberstkatid2132560-1
eldorado.secondarypublicationtrue

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