Assessment of trimethylamine-N-oxide at the blood-cerebrospinal fluid barrier

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dc.contributor.authorEnko, Dietmar
dc.contributor.authorZelzer, Sieglind
dc.contributor.authorNiedrist, Tobias
dc.contributor.authorHolasek, Sandra
dc.contributor.authorBaranyi, Andreas
dc.contributor.authorSchnedl, Wolfgang J.
dc.contributor.authorHerrmann, Markus
dc.contributor.authorMeinitzer, Andreas
dc.date.accessioned2020-12-14T14:52:32Z
dc.date.available2020-12-14T14:52:32Z
dc.date.issued2020-09-09
dc.description.abstractRecently, the microbiome-derived trimethylamine-N-oxide (TMAO) was shown to be present in human cerebrospinal fluid (CSF). However, data on the potential of TMAO crossing the blood-CSF barrier are still lacking. This retrospective study aimed at investigating possible associations between the CSF/serum albumin (QALB) and TMAO (QTMAO) quotient and evaluating QTMAO values in individuals with and without blood-CSF barrier dysfunction. A total of 290 patients, who underwent diagnostic lumbar puncture with QALB and QTMAO determination, were evaluated. Serum and CSF TMAO measurements were performed on a tandem mass spectrometry SCIEX QTRAP 4500 (Applied Biosystems, Framingham, MA, USA) coupled with an Agilent 1260 Infinity HPLC system (Agilent Technologies Santa Clara, CA, USA). Serum and CSF albumin were measured on the Atellica® NEPH 630 system (Siemens Healthineers, Erlangen, Germany). CSF TMAO levels were positively correlated with serum TMAO levels (ρ = 0.709, p < 0.001). The QALB was significantly associated with the QTMAO (ß-coefficient = 0.312; p < 0.001). A total of 117 patients with blood-CSF barrier dysfunction had significantly higher median (Q1 – Q3) QTMAO values (4.7 (2.8 – 7.5) vs. 3.8 (2.5 – 5.7) x 10-1, p = 0.002) compared to 173 individuals with normal blood-CSF barrier function. CSF and serum TMAO concentrations were significantly associated in 290 CSF/serum pairs from lumbar punctures of clinical routine. QALB showed a relevant influence on QTMAO. Present results indicate that TMAO may cross the blood-CSF barrier.en
dc.identifier.citationEnko, D., Zelzer, S., Niedrist, T., Holasek, S., Baranyi, A., Schnedl, W. J. ., Herrmann, M., & Meinitzer, A. (2020). Assessment of trimethylamine-N-oxide at the blood-cerebrospinal fluid barrier: Results from 290 lumbar punctures. EXCLI Journal, 19, 1275-1281. https://doi.org/10.17179/excli2020-276de
dc.identifier.issn1611-2156
dc.identifier.urihttp://hdl.handle.net/2003/39891
dc.identifier.urihttp://dx.doi.org/10.17877/DE290R-21781
dc.language.isoen
dc.publisherIfADo - Leibniz Research Centre for Working Environment and Human Factors, Dortmundde
dc.relation.ispartofseriesEXCLI Journal;Vol. 19. 2020, pp.1275-1281
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectBiomarkersen
dc.subjectBlood-cerebrospinal fluid barrieren
dc.subjectLiquid chromatography-tandem mass spectrometryen
dc.subjectTrimethylamine-N-oxideen
dc.subject.ddc610
dc.titleAssessment of trimethylamine-N-oxide at the blood-cerebrospinal fluid barrieren
dc.title.alternativeresults from 290 lumbar puncturesen
dc.typeText
dc.type.publicationtypearticle
dcterms.accessRightsopen access
eldorado.dnb.zdberstkatid2132560-1
eldorado.identifier.urlhttps://www.excli.de/index.php/excli/article/view/2763
eldorado.secondarypublicationtrue

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