Stage-specific expressions of four different ribonuclease H genes in Leishmania

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dc.contributor.authorChaudhuri, Gautamde
dc.contributor.authorFarrow, Anitra L.de
dc.contributor.authorMisra, Smitade
dc.date.accessioned2008-06-17T13:57:40Z
dc.date.available2008-06-17T13:57:40Z
dc.date.issued2005-07-13de
dc.description.abstractThe human pathogen of the genus Leishmania cause worldwide morbidity and infection of visceral organs by some species may be lethal. Lack of rational chemotherapy against these pathogens dictates the study of differential biochemistry and molecular biology of the parasite as compared to its human host. The ubiquitous enzyme ribonuclease H (RNase H, EC 3.1.26.4) cleaves the RNA from a RNA:DNA duplex and is critical for the replication of DNA in the nucleus and the mitochondria. We have characterized four RNase H genes from Leishmania: one is of type I (LRNase HI) and three others are of type II (LRNase HIIA, -HIIB and -HIIC). In contrast human cells have only one type I and one type II RNase H. All the four RNase H genes in Leishmania are single copy and located in discrete chromosomes. When expressed inside RNase H-deficient E. coli, all of the four Leishmania RNase H were capable to complement the genetic defect of the E. coli, indicating their identity as RNase H. The enzymes are differentially expressed in the promastigotes and the amastigotes, the forms that thrives in entirely different physico-chemical conditions in nature. Nucleotide sequences of the 5'-UTRs of three of these mRNAs have upstream open reading frames. Understanding the regulation of these four distinct ribonuclease H genes in Leishmania will help us better understand leishmanial parasitism and may help us to design rational chemotherapy against the pathogen.en
dc.identifier.issn1611-2156de
dc.identifier.urihttp://hdl.handle.net/2003/25649
dc.identifier.urihttp://dx.doi.org/10.17877/DE290R-341
dc.language.isoende
dc.relation.ispartofseriesEXCLI Journal ; Vol. 4, 2005en
dc.subjectAmastigoteen
dc.subjectLeishmania majoren
dc.subjectPromastigotesen
dc.subjectRibonuclease Hen
dc.subjectTrypanosoma bruceien
dc.subjectTrypanosoma cruzien
dc.subjectUpstream open reading frameen
dc.subject.ddc610
dc.titleStage-specific expressions of four different ribonuclease H genes in Leishmaniaen
dc.typeTextde
dc.type.publicationtypearticlede
dcterms.accessRightsopen access
eldorado.dnb.zdberstkatid2132560-1

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