Identification of circulating miRNA profiles that distinguish malignant pleural mesothelioma from lung adenocarcinoma

Simple item page
dc.contributor.authorGayosso - Gómez, L. V.
dc.contributor.authorZárraga - Granados, G.
dc.contributor.authorParedes - Garcia, P.
dc.contributor.authorFalfán - Valencia, R.
dc.contributor.authorVazquez - Manríquez, M. E.
dc.contributor.authorMartinez - Barrera, L. M.
dc.contributor.authorCastillo - Gonzalez, P.
dc.contributor.authorRumbo - Nava, U.
dc.contributor.authorGuevara - Gutierrez, R.
dc.contributor.authorRivera - Bravo, B.
dc.contributor.authorRamirez - Venegas, A.
dc.contributor.authorSansores, R.
dc.contributor.authorNegrete - Garcia, M. C.
dc.contributor.authorOrtiz - Quintero, B.
dc.date.accessioned2014-11-25T13:14:19Z
dc.date.available2014-11-25T13:14:19Z
dc.date.issued2014-07-08
dc.description.abstractAccurate diagnosis of malignant pleura mesothelioma (MPM) is challenging. Differential diagnosis of MPM versus lung adenocarcinoma (AD) is particularly difficult, yet clinically important since the two neoplasias call for different treatment approaches. Circulating miRNA-profiling to identify miRNAs that can be used to distinguish MPM from AD has not been reported. We conducted a wide screening study of miRNA profiles in serum pools of MPM patients (N = 11), AD patients (N = 36), and healthy subjects (N = 45) to identify non-invasive biomarkers for differential diagnosis of MPM and AD, using deep sequencing. Sequencing detected up to 300 known miRNAs and up to 25 novel miRNAs species in the serum samples. Among known miRNAs, 7 were upregulated in MPM and 12 were upregulated in AD compared to healthy controls. Of these, eight were distinctive for AD and three were unique for MPM. Direct comparison of the miRNA profiles for MPM and AD revealed differences in miRNA levels that could be useful for differential diagnosis. No differentially expressed novel miRNAs were found. Further bioinformatics analysis indicated that three upregulated miRNAs in MPM are associated with the p38 pathway. There are unique alterations in serum miRNAs in MPM and AD compared to healthy controls, as well as differences between MPM and AD profiles. Differing miRNA levels between MPM and AD may be useful for differential diagnosis. A potential association to p38 pathway of three upregulated miRNAs in MPM was revealed.en
dc.identifier.issn1611-2156
dc.identifier.urihttp://hdl.handle.net/2003/33707
dc.identifier.urihttp://dx.doi.org/10.17877/DE290R-6925
dc.language.isoen
dc.relation.ispartofseriesEXCLI Journal ; Vol. 13, 2014en
dc.subjectCirculating miRNAsen
dc.subjectmalignant pleural mesotheliomaen
dc.subjectlung adenocarcinomaen
dc.subjectdifferential diagnosisen
dc.subjectnon - invasive biomarkersen
dc.subject.ddc610
dc.titleIdentification of circulating miRNA profiles that distinguish malignant pleural mesothelioma from lung adenocarcinomaen
dc.typeText
dc.type.publicationtypearticle
dcterms.accessRightsopen access
eldorado.dnb.zdberstkatid2132560-1

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Ortiz-Quintero_08072014_proof.pdf
Size:
453.82 KB
Format:
Adobe Portable Document Format
Description:
DNB
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.85 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Original Articles