Enhanced interleukin-10 signaling with 14-member macrolides in lipopolysaccharide-stimulated macrophages
| dc.contributor.author | Abe, Shuichi | |
| dc.contributor.author | Inoue, Sumito | |
| dc.contributor.author | Ishikawa, Tomomi | |
| dc.contributor.author | Kubota, Isao | |
| dc.contributor.author | Shibata, Yoko | |
| dc.contributor.author | Takabatake, Noriaki | |
| dc.contributor.author | Yamauchi, Keiko | |
| dc.date.accessioned | 2008-10-21T12:42:53Z | |
| dc.date.available | 2008-10-21T12:42:53Z | |
| dc.date.issued | 2008-10-21T12:42:53Z | |
| dc.description.abstract | Immunomodulatory effects of 14-member macrolides, namely erythromycin (EM) and clarithromycin (CAM), have been reported in chronic respiratory infectious diseases. It has been suggested that 14-member macrolides have immunomodulatory effects on various lung cells such as alveolar macrophages. Interleukin (IL)-10 is an immunomodulatory cytokine that performs an irreplaceable role in negatively regulating inflammation, primarily via a mechanism that selectively blocks the expression of pro-inflammatory genes. It activates sig-nal transducer and activator of transcription (STAT)-3, and subsequently induces the suppres-sor of cytokine signaling-3 (SOCS-3), resulting in the resolution of inflammatory response in macrophages. However, it has been still unclear whether 14-member macrolides exert immu-nomodulatory effects via IL-10 signaling pathway. We aimed to evaluate whether 14-member macrolides affect the IL-10 signaling pathway. The RAW264.7 macrophage cell line was pre-treated with EM or CAM, and stimulated with lipopolysaccharide (LPS). The levels of IL-10, IL-10 receptor, phosphorylated (p) STAT-3, and SOCS-3 were determined by RT-PCR, ELISA and immunoblotting. We observed increased levels of IL-10, p-STAT-3 and SOCS-3 in the treated cells. In addition, while the levels of tumor necrosis factor-α 6 h after LPS stimulation was equal between vehicle-treated and CAM-treated macrophage cells, those of CAM-treated cells were repressed 36 h following LPS stimulation, compared with those of the control cells. Therefore, the 14-member macrolides may initiate an early resolution of inflammation, in part, via the enhancement of the IL-10/STAT-3/SOCS-3 pathway. | en |
| dc.identifier.issn | 1611-2156 | |
| dc.identifier.uri | http://hdl.handle.net/2003/25810 | |
| dc.identifier.uri | http://dx.doi.org/10.17877/DE290R-576 | |
| dc.language.iso | en | de |
| dc.relation.ispartofseries | EXCLI Journal ; Vol. 7, 2008 | en |
| dc.subject | clarithromycin | en |
| dc.subject | erythromycin | en |
| dc.subject | immunomodulatory effects | en |
| dc.subject | interleukin-10 | de |
| dc.subject | macrophage | en |
| dc.subject | SOCS-3 | de |
| dc.subject | STAT3 | de |
| dc.subject.ddc | 610 | |
| dc.title | Enhanced interleukin-10 signaling with 14-member macrolides in lipopolysaccharide-stimulated macrophages | en |
| dc.type | Text | de |
| dc.type.publicationtype | article | en |
| dcterms.accessRights | open access | |
| eldorado.dnb.zdberstkatid | 2132560-1 |