Efficacy of Caltropis procera and Ficus sycomorus extracts in treating MRSA (methicillin-resistant Staphylococcus aureus)-keratitis in rabbit
dc.contributor.author | Sayed, Waiel F. | |
dc.contributor.author | Salem, Wesam M. A. | |
dc.contributor.author | Haridy, Mohie A. M. | |
dc.contributor.author | Hassan, Ne’mat H. | |
dc.date.accessioned | 2016-05-23T09:16:40Z | |
dc.date.available | 2016-05-23T09:16:40Z | |
dc.date.issued | 2015-06-16 | |
dc.description.abstract | MRSA-induced keratitis in rabbit was used to evaluate the therapeutic effect of F. sycomorus leaves and C. procera latex extracts. Within the 6 rabbit groups tested, group 1 received sterilized saline, while other groups (2 to 6) received 100 μl of intrastromal injections of 1.5×103 colony forming unit (cfu) ml-1 of methicillin-resistant Staphylococcus aureus (MRSA). After 12 hours, groups 3 to 6 also received chloramphenicol, aqueous extract of C. procera latex, aqueous and alcoholic extracts of F. sycomorus leaves, respectively 3 times daily for 12 successive days. The tested extracts inhibited MRSA growth in vitro (i.e. on culture medium). Colony counts in cornea discs from groups 3 to 6 were significantly reduced (P ≤ 0.001) compared to group 2 (untreated). Clinical signs of keratitis were observed on group 2 until the end of experiment. In groups 3 to 6, gradual recovery was observed and signs disappeared by the 12th DPI (days post inoculation). Only mild symptoms persisted in group 5 (aqueous extract of leaves). In group 3 and 5, cornea, iris, ciliary body and conjunctiva showed mild leukocytic infiltration and depigmentation of melanin cells while recovery of cornea and iris was observed in groups 4 and 6. In conclusion, the used extracts have potential therapeutic effects on MRSA-induced keratitis in rabbit. | en |
dc.identifier.doi | 10.17179/excli2015-350 | |
dc.identifier.issn | 1611-2156 | |
dc.identifier.uri | http://hdl.handle.net/2003/35009 | |
dc.identifier.uri | http://dx.doi.org/10.17877/DE290R-17057 | |
dc.language.iso | en | |
dc.relation.ispartofseries | EXCLI Journal;Vol. 14, 2015 | en |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.subject | Ficus sycomorus | en |
dc.subject | Caltropis procera | en |
dc.subject | latex | en |
dc.subject | Staphylococcus aureus MRSA | en |
dc.subject | keratitis | de |
dc.subject.ddc | 610 | |
dc.title | Efficacy of Caltropis procera and Ficus sycomorus extracts in treating MRSA (methicillin-resistant Staphylococcus aureus)-keratitis in rabbit | en |
dc.type | Text | |
dc.type.publicationtype | article | |
dcterms.accessRights | open access | |
eldorado.dnb.zdberstkatid | 2132560-1 |
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