Efficacy of Caltropis procera and Ficus sycomorus extracts in treating MRSA (methicillin-resistant Staphylococcus aureus)-keratitis in rabbit

Sayed, Waiel F.; Salem, Wesam M. A.; Haridy, Mohie A. M.; Hassan, Ne’mat H.

Efficacy of Caltropis procera and Ficus sycomorus extracts in treating MRSA (methicillin-resistant Staphylococcus aureus)-keratitis in rabbit

dc.contributor.author	Sayed, Waiel F.
dc.contributor.author	Salem, Wesam M. A.
dc.contributor.author	Haridy, Mohie A. M.
dc.contributor.author	Hassan, Ne’mat H.
dc.date.accessioned	2016-05-23T09:16:40Z
dc.date.available	2016-05-23T09:16:40Z
dc.date.issued	2015-06-16
dc.description.abstract	MRSA-induced keratitis in rabbit was used to evaluate the therapeutic effect of F. sycomorus leaves and C. procera latex extracts. Within the 6 rabbit groups tested, group 1 received sterilized saline, while other groups (2 to 6) received 100 μl of intrastromal injections of 1.5×103 colony forming unit (cfu) ml-1 of methicillin-resistant Staphylococcus aureus (MRSA). After 12 hours, groups 3 to 6 also received chloramphenicol, aqueous extract of C. procera latex, aqueous and alcoholic extracts of F. sycomorus leaves, respectively 3 times daily for 12 successive days. The tested extracts inhibited MRSA growth in vitro (i.e. on culture medium). Colony counts in cornea discs from groups 3 to 6 were significantly reduced (P ≤ 0.001) compared to group 2 (untreated). Clinical signs of keratitis were observed on group 2 until the end of experiment. In groups 3 to 6, gradual recovery was observed and signs disappeared by the 12th DPI (days post inoculation). Only mild symptoms persisted in group 5 (aqueous extract of leaves). In group 3 and 5, cornea, iris, ciliary body and conjunctiva showed mild leukocytic infiltration and depigmentation of melanin cells while recovery of cornea and iris was observed in groups 4 and 6. In conclusion, the used extracts have potential therapeutic effects on MRSA-induced keratitis in rabbit.	en
dc.identifier.doi	10.17179/excli2015-350
dc.identifier.issn	1611-2156
dc.identifier.uri	http://hdl.handle.net/2003/35009
dc.identifier.uri	http://dx.doi.org/10.17877/DE290R-17057
dc.language.iso	en
dc.relation.ispartofseries	EXCLI Journal;Vol. 14, 2015	en
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/
dc.subject	Ficus sycomorus	en
dc.subject	Caltropis procera	en
dc.subject	latex	en
dc.subject	Staphylococcus aureus MRSA	en
dc.subject	keratitis	de
dc.subject.ddc	610
dc.title	Efficacy of Caltropis procera and Ficus sycomorus extracts in treating MRSA (methicillin-resistant Staphylococcus aureus)-keratitis in rabbit	en
dc.type	Text
dc.type.publicationtype	article
dcterms.accessRights	open access
eldorado.dnb.zdberstkatid	2132560-1