Efficacy of Caltropis procera and Ficus sycomorus extracts in treating MRSA (methicillin-resistant Staphylococcus aureus)-keratitis in rabbit

dc.contributor.authorSayed, Waiel F.
dc.contributor.authorSalem, Wesam M. A.
dc.contributor.authorHaridy, Mohie A. M.
dc.contributor.authorHassan, Ne’mat H.
dc.date.accessioned2016-05-23T09:16:40Z
dc.date.available2016-05-23T09:16:40Z
dc.date.issued2015-06-16
dc.description.abstractMRSA-induced keratitis in rabbit was used to evaluate the therapeutic effect of F. sycomorus leaves and C. procera latex extracts. Within the 6 rabbit groups tested, group 1 received sterilized saline, while other groups (2 to 6) received 100 μl of intrastromal injections of 1.5×103 colony forming unit (cfu) ml-1 of methicillin-resistant Staphylococcus aureus (MRSA). After 12 hours, groups 3 to 6 also received chloramphenicol, aqueous extract of C. procera latex, aqueous and alcoholic extracts of F. sycomorus leaves, respectively 3 times daily for 12 successive days. The tested extracts inhibited MRSA growth in vitro (i.e. on culture medium). Colony counts in cornea discs from groups 3 to 6 were significantly reduced (P ≤ 0.001) compared to group 2 (untreated). Clinical signs of keratitis were observed on group 2 until the end of experiment. In groups 3 to 6, gradual recovery was observed and signs disappeared by the 12th DPI (days post inoculation). Only mild symptoms persisted in group 5 (aqueous extract of leaves). In group 3 and 5, cornea, iris, ciliary body and conjunctiva showed mild leukocytic infiltration and depigmentation of melanin cells while recovery of cornea and iris was observed in groups 4 and 6. In conclusion, the used extracts have potential therapeutic effects on MRSA-induced keratitis in rabbit.en
dc.identifier.doi10.17179/excli2015-350
dc.identifier.issn1611-2156
dc.identifier.urihttp://hdl.handle.net/2003/35009
dc.identifier.urihttp://dx.doi.org/10.17877/DE290R-17057
dc.language.isoen
dc.relation.ispartofseriesEXCLI Journal;Vol. 14, 2015en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectFicus sycomorusen
dc.subjectCaltropis proceraen
dc.subjectlatexen
dc.subjectStaphylococcus aureus MRSAen
dc.subjectkeratitisde
dc.subject.ddc610
dc.titleEfficacy of Caltropis procera and Ficus sycomorus extracts in treating MRSA (methicillin-resistant Staphylococcus aureus)-keratitis in rabbiten
dc.typeText
dc.type.publicationtypearticle
dcterms.accessRightsopen access
eldorado.dnb.zdberstkatid2132560-1

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