Metformin restores the mitochondrial membrane potentials in association with a reduction in TIMM23 and NDUFS3 in MPP+-induced neurotoxicity in SH-SY5Y cells

Chanthammachat, Pitak; Dharmasaroja, Permphan

Metformin restores the mitochondrial membrane potentials in association with a reduction in TIMM23 and NDUFS3 in MPP+-induced neurotoxicity in SH-SY5Y cells

dc.contributor.author	Chanthammachat, Pitak
dc.contributor.author	Dharmasaroja, Permphan
dc.date.accessioned	2020-03-06T13:37:56Z
dc.date.available	2020-03-06T13:37:56Z
dc.date.issued	2019-09-10
dc.description.abstract	SH - SY5Y cells exposed to l - methyl - 4 - phenylpyridinium (MPP + ) develop mitochondrial dys- function and other cellular responses similar to th ose that occur in the dopaminergic neurons of patients with Parkinson ’ s disease (PD) . It has been shown in animal models of PD that neuronal death can be prevented by metformin, an anti - diabetic drug . Both MPP + and metformin inhibit complex I of the mitochondrial resp iratory chain. It has been reported that decreased levels of the mitochondrial inner membrane proteins TI MM23 and NDUFS3 are associated with the in- creased generation of reactive oxygen species and mitochondrial de polarization. In the present study, we investigated the e ffects of metformin on MPP + - induced neurotoxicity using differen- tiated human SH - SY5Y neuroblastoma cells . The results showed that pretreatment with met- formin increased the viability of MPP + - treated SH - SY5Y cells. Pretreatment with metformin decreased the expr ession of TIMM23 and NDUFS3 in MPP + - treated SH - SY5Y cells. This was correlated with reduced mitochondrial fragmenta tion and an improvement in the mitochondrial membrane potential . These results suggest that metformin pretreatment protects against MPP + - induced neurotoxicity, and offer insights into th e potential role of me tformin in protecting against toxin - induced parkinsonism	en
dc.identifier.issn	1611-2156
dc.identifier.uri	http://hdl.handle.net/2003/39050
dc.identifier.uri	http://dx.doi.org/10.17877/DE290R-20969
dc.language.iso	en
dc.relation.ispartofseries	EXCLI Journal;Vol. 18 2019
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/
dc.subject	MPP +	en
dc.subject	Metformin	en
dc.subject	Mitochondria	en
dc.subject	SH - SY5Y cells	en
dc.subject	Parkinson ’ s disease	en
dc.subject.ddc	610
dc.title	Metformin restores the mitochondrial membrane potentials in association with a reduction in TIMM23 and NDUFS3 in MPP+-induced neurotoxicity in SH-SY5Y cells	en
dc.type	Text
dc.type.publicationtype	article
dcterms.accessRights	open access
eldorado.dnb.zdberstkatid	2132560-1
eldorado.secondarypublication	true