The Absence of Prion-Like Infectivity in Mice expressing Prion Protein-Like Protein

Atarashi, Ryuichiro; Katamine, Shigeru; Sakaguchi, Suehiro; Shigematsu, Kazuto

The Absence of Prion-Like Infectivity in Mice expressing Prion Protein-Like Protein

dc.contributor.author	Atarashi, Ryuichiro	de
dc.contributor.author	Katamine, Shigeru	de
dc.contributor.author	Sakaguchi, Suehiro	de
dc.contributor.author	Shigematsu, Kazuto	de
dc.date.accessioned	2008-06-17T13:46:42Z
dc.date.available	2008-06-17T13:46:42Z
dc.date.issued	2004-10-28	de
dc.description.abstract	Cellular prion protein, PrP^C, undergoes pathogenic structural conversion into the proteinase K (PK)-resistant isoform, PrP^Sc, to constitute a nucleic acid-free infectious agent, so called a prion. To determine whether a recently identified PrP-like protein, named PrPLP/Dpl, could also be transformed to a prion-like protein, we intracerebrally inoculated a mouse-adapted Fukuoka-1 prion into Ngsk and Zrch I mice either homozygously (Prnp^0/0) or heterozygously (Prnp^0/+) devoid of PrP^C. Only the former expressed PrPLP/Dpl ectopically in the brains, particularly in neurons. Ngsk Prnp^0/+ and Zrch I Prnp^0/+ mice similarly developed the disease. The diseased Ngsk Prnp0/+ mice transmitted the disease to the mice expressing PrP^C but not to the mice expressing PrPLP/Dpl, showing abundant accumulation of PrP^Sc but not PK-resistant PrPLP/Dpl in the brains. Moreover, the inoculated Ngsk Prnp^0/0 mice neither developed the disease nor produced any infectivity transmissible to PrPLP/Dpl-expressing mice. These results indicate that PrPLP/Dpl have no potential to undergo pathogenic conversion to form a prion-like infectious particle.	en
dc.identifier.issn	1611-2156	de
dc.identifier.uri	http://hdl.handle.net/2003/25639
dc.identifier.uri	http://dx.doi.org/10.17877/DE290R-8160
dc.language.iso	en	de
dc.relation.ispartofseries	EXCLI Journal ; Vol. 3, 2004	en
dc.subject	knockout mice	en
dc.subject	prion	en
dc.subject	prion protein	en
dc.subject	prion protein-like protein	en
dc.subject.ddc	610
dc.title	The Absence of Prion-Like Infectivity in Mice expressing Prion Protein-Like Protein	en
dc.type	Text	de
dc.type.publicationtype	article	de
dcterms.accessRights	open access
eldorado.dnb.zdberstkatid	2132560-1

Files

Original bundle

Now showing 1 - 1 of 1

Name:: Sakaguchi11-04proofrev.pdf
Size:: 186.99 KB
Format:: Adobe Portable Document Format
Description:: DNB

Download

Collections

Original Articles