Morphological and genetic characteristics of Nicotiana langsdorffii, N.
glauca and its hybrid

Han, Woong; Heo, Kweon; Jin, Ying-Shan; Lim, Hak-Tae; Wang, Myeong-Hyeon

Morphological and genetic characteristics of Nicotiana langsdorffii, N. glauca and its hybrid

dc.contributor.author	Han, Woong	de
dc.contributor.author	Heo, Kweon	de
dc.contributor.author	Jin, Ying-Shan	de
dc.contributor.author	Lim, Hak-Tae	de
dc.contributor.author	Wang, Myeong-Hyeon	de
dc.date.accessioned	2008-06-17T13:57:19Z
dc.date.available	2008-06-17T13:57:19Z
dc.date.issued	2006-07-04	de
dc.description.abstract	Plant tumors, including genetic tumors, are disorganized and proliferate in an uncontrolled fashion. In this report we describe the morphological, physiological and genetic properties of Nicotiana. langsdorffii and Nicotiana. glauca and their hybrids (Nicotiana. langsdorffii x Nicotiana. Glauca). Nicotiana. langsdorffii leaves are oblate and pubescent with winged petioles, while Nicotiana. glauca leaf-blades are rubbery and oval-to-heart-shaped. The hybrid plants are intermediate in leaf shape, with anisocytic stomata and well-developed trichomes. In addition, they produce tumors in the absence of bacteria and exogenous hormones. Tumor growth in the hybrid plants was not affected by indole-3-acetic acid or kinetin. Genetic polymorphism was analyzed by the randomly amplified polymorphic DNA technique in the parents (Nicotiana. langsdorffii and Nicotiana. glauca) and in the genetic tumors produced by the hybrids. A total of 128 randomly amplified polymorphic DNA fragments were scored from fifteen random primers, and pronounced differences were found between the genetic tumors and their parents. These observations show that randomly amplified polymorphic DNA markers are might informative about genetic similarities and dissimilarities.	en
dc.identifier.issn	1611-2156	de
dc.identifier.uri	http://hdl.handle.net/2003/25648
dc.identifier.uri	http://dx.doi.org/10.17877/DE290R-8183
dc.language.iso	en	de
dc.relation.ispartofseries	EXCLI Journal ; Vol. 4, 2005	en
dc.subject	auxin	en
dc.subject	callus	en
dc.subject	cytokinin	en
dc.subject	genetic tumors	en
dc.subject	hybrid plant	en
dc.subject	Nicotiana glauca	en
dc.subject	Nicotiana langsdorffii	en
dc.subject	randomly amplified polymorphic DNA	en
dc.subject.ddc	610
dc.title	Morphological and genetic characteristics of Nicotiana langsdorffii, N. glauca and its hybrid	en
dc.type	Text	de
dc.type.publicationtype	article	de
dcterms.accessRights	open access
eldorado.dnb.zdberstkatid	2132560-1

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