Electro-pharmacological profiles of two brain mitoplast anion channels

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dc.contributor.authorFahanik-Babaei, Javad
dc.contributor.authorShayanfa, Farzad
dc.contributor.authorKhodaee, Naser
dc.contributor.authorSaghiri, Reza
dc.contributor.authorEliassi, Afsaneh
dc.date.accessioned2017-10-26T11:34:48Z
dc.date.available2017-10-26T11:34:48Z
dc.date.issued2017-04-18
dc.description.abstractWe have characterized the conduction and blocking properties of two different chloride channels from brain mi- tochondrial inner membranes after incorporation into planar lipid bilayers. Our experiments revealed the existence of channels with a mean conductance of 158 ± 7 and 301 ± 8 pS in asymmetrical 200 mM cis/50 mM trans KCl solutions. We determined that the channels were ten times more permeable for Cl− than for K+, calculated from the reversal potential using the Goldman-Hodgkin-Katz equation. The channels were bell-shaped voltage depend- ent, with maximum open probability 0.9 at ± 20 mV. Two mitochondrial chloride channels were blocked after the addition of 10 µM DIDS. In addition, 158 pS chloride channel was blocked by 300 nM NPPB, acidic pH and 2.5 mM ATP, whereas the 301 pS chloride channel was blocked by 600 µM NPPB but not by acidic pH or ATP. Gating and conducting behaviors of these channels were unaffected by Ca2+. These results demonstrate that the inner membrane, is probably identical to IMAC and 301 pS Cl channel displays different properties than those classically described for mitochondrial anion channels.de
dc.identifier.issn1611-2156
dc.identifier.urihttp://hdl.handle.net/2003/36146
dc.identifier.urihttp://dx.doi.org/10.17877/DE290R-18162
dc.language.isoen
dc.relation.ispartofseriesEXCLI Journal;Vol. 16 2017
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectmitochondriade
dc.subjectchloride channelsde
dc.subjectsingle channelde
dc.subjectintracellular ion channelsde
dc.subjectbrainde
dc.subjectmitoplastde
dc.subject.ddc610
dc.titleElectro-pharmacological profiles of two brain mitoplast anion channelsde
dc.title.alternativeinferences from single channel recordingde
dc.typeText
dc.type.publicationtypearticle
dcterms.accessRightsopen access
eldorado.dnb.zdberstkatid2132560-1
eldorado.secondarypublicationtrue

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