Autor(en): | Bai, Hongzhi Wang, Hui Sun, Junde Irfan, Muhammad Han, Mei Huang, Yuqian Han, Xiaori Yang, Qian |
Titel: | Production, purification and characterization of novel beta glucosidase from newly isolated Penicillium simplicissimum H-11 in submerged fermentation |
Sprache (ISO): | en |
Zusammenfassung: | β-Glucosidase is an important component of the cellulase complex. It not only hydrolyzes cellobiose and short-chain cellooligos accharides to glucose, but also removes the inhibitory effect of cellobiose on the β-1, 4-endoglucanase and exoglucanase, thereby increasing the overall rate of cellulose biodegradation. β-glucosidasefrom culture supernatant of a fungus Penicillium simplicissimum was purified to homogeneity, by using ammonium sulfate fraction, Sephadex G-100 chromatography, and its properties were studied. The molecular mass of the enzyme was about 126.0 kDa, as identified by 12% SDS-PAGE. The optimum pH and temperature were 4.4 ~ 5.2 and 60°C, respectively. The enzyme was stable in pH 5.2 ~ 6.4 and under 40°C. Metal profile of the enzyme showed that Mn2+ enhances its activity, while Cu2+, Co2+ and Fe3+ cause obvious inhibition. The Km and Vmax was 14.881 mg/ml and 0.364 mg ml/min against salicin as a Substrate. This enzyme had secondary protein structure as evidenced by FTIR spectrum. |
Schlagwörter: | β -Glucosidase Penicillium simplicissimum purification characterization |
URI: | http://hdl.handle.net/2003/32936 http://dx.doi.org/10.17877/DE290R-7346 |
Erscheinungsdatum: | 2013-06-13 |
Enthalten in den Sammlungen: | Original Articles |
Dateien zu dieser Ressource:
Datei | Beschreibung | Größe | Format | |
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Bai_13062013_proof.pdf | DNB | 344.76 kB | Adobe PDF | Öffnen/Anzeigen |
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