Autor(en): | Sayed, Waiel F. Salem, Wesam M. A. Haridy, Mohie A. M. Hassan, Ne’mat H. |
Titel: | Efficacy of Caltropis procera and Ficus sycomorus extracts in treating MRSA (methicillin-resistant Staphylococcus aureus)-keratitis in rabbit |
Sprache (ISO): | en |
Zusammenfassung: | MRSA-induced keratitis in rabbit was used to evaluate the therapeutic effect of F. sycomorus leaves and C. procera latex extracts. Within the 6 rabbit groups tested, group 1 received sterilized saline, while other groups (2 to 6) received 100 μl of intrastromal injections of 1.5×103 colony forming unit (cfu) ml-1 of methicillin-resistant Staphylococcus aureus (MRSA). After 12 hours, groups 3 to 6 also received chloramphenicol, aqueous extract of C. procera latex, aqueous and alcoholic extracts of F. sycomorus leaves, respectively 3 times daily for 12 successive days. The tested extracts inhibited MRSA growth in vitro (i.e. on culture medium). Colony counts in cornea discs from groups 3 to 6 were significantly reduced (P ≤ 0.001) compared to group 2 (untreated). Clinical signs of keratitis were observed on group 2 until the end of experiment. In groups 3 to 6, gradual recovery was observed and signs disappeared by the 12th DPI (days post inoculation). Only mild symptoms persisted in group 5 (aqueous extract of leaves). In group 3 and 5, cornea, iris, ciliary body and conjunctiva showed mild leukocytic infiltration and depigmentation of melanin cells while recovery of cornea and iris was observed in groups 4 and 6. In conclusion, the used extracts have potential therapeutic effects on MRSA-induced keratitis in rabbit. |
Schlagwörter: | Ficus sycomorus Caltropis procera latex Staphylococcus aureus MRSA keratitis |
URI: | http://hdl.handle.net/2003/35009 http://dx.doi.org/10.17877/DE290R-17057 |
Erscheinungsdatum: | 2015-06-16 |
Rechte (Link): | http://creativecommons.org/licenses/by/4.0/ |
Enthalten in den Sammlungen: | Original Articles |
Dateien zu dieser Ressource:
Datei | Beschreibung | Größe | Format | |
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Sayed_16062015_supplementary_material.pdf | DNB | 1.07 MB | Adobe PDF | Öffnen/Anzeigen |
Sayed_16062015_proof.pdf | DNB | 282.69 kB | Adobe PDF | Öffnen/Anzeigen |
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