Authors: Kaplanow, Irene
Görzgen, Fabian
Merz, Juliane
Schembecker, Gerhard
Title: Mass transfer of proteins in aqueous two-phase systems
Language (ISO): en
Abstract: Aqueous Two-Phase Extraction is known to be a gentle separation technique for biochemical molecules where product partitioning is fast. However, the reason for the high mass transfer rates has not been investigated, yet. Many researchers claim that the low interfacial tension facilitates the formation of very small droplets and with it a large interfacial area causing a fast partitioning. However, an experimental evidence for this hypothesis has not been published yet. In this study, the mass transfer coefficients of two proteins, namely lysozyme and bromelain, were determined by providing a defined interfacial area for partitioning. Compared to low molecular weight solutes the mass transfer coefficient for the proteins investigated was small proving for the first time that the large interfacial area and not fast diffusion seems to be the reason for fast protein partitioning.
Subject Headings (RSWK): Extraktion
Issue Date: 2019-03-06
Rights link:
Appears in Collections:Lehrstuhl Anlagen- und Prozesstechnik

Files in This Item:
File Description SizeFormat 
article.pdf1.39 MBAdobe PDFView/Open

This item is protected by original copyright

This item is licensed under a Creative Commons License Creative Commons