Full metadata record
DC FieldValueLanguage
dc.contributor.authorHalka, Lisa-
dc.contributor.authorWichmann, Rolf-
dc.description.abstractFusicocca-2,10(14)-diene (FCdiene) is a tricyclic diterpene which has many pharmaceutical applications, for example, it is a precursor for different anticancer drugs, including fusicoccin A. Chemical synthesis of this diterpene is not economical as it requires 14 steps with several stereospecific reactions. FCdiene is naturally produced at low titers in phytopathogenic filamentous fungi. However, production of FCdiene can be achieved via expression of fusicoccadiene synthase in yeast. The objective of this study is to increase FCdiene production by optimizing the yeast fermentation process. Our preliminary fermentations showed influences of carbon sources, buffer agents, and oxygen supply on FCdiene production. Buffer agents as well as oxygen supply were investigated in detail at 0.2 and 1.8 L cultivation volumes. Using glucose as the carbon source, FCdiene concentrations were increased to 240 mgFCdiene/L by optimizing pH and oxygen conditions. In situ extraction and adsorption techniques were examined at the 0.2 L scale to determine if these techniques could improve FCdiene yields. Different adsorbents and solvents were tested with in situ product recovery and 4-fold increases in FCdiene productivity could be shown. The results generated in this work provide a proof-of-concept for the fermentative production of FCdiene from S. cerevisiae as a practical alternative to chemical synthesis.de
dc.relation.ispartofseriesFermentation;Jg. 4, Heft: 3 | S. 65-1-65-12-
dc.subjectDissolved oxygen tensionde
dc.subjectLab-scale fermentationde
dc.subjectIn situ product recoveryde
dc.subjectProduction conceptde
dc.titleEnhanced production and in situ product recovery of fusicocca-2,10(14)-diene from yeastde
dc.subject.rswkProzessentwicklung <Technik>de
dcterms.accessRightsopen access-
eldorado.secondarypublication.primarycitationFermentation : Jg. 4. 2018, Heft: 3 | S. 65-1-65-12de
Appears in Collections:Arbeitsgruppe Bioverfahrenstechnik

Files in This Item:
File Description SizeFormat 
fermentation-04-00065.pdf1.12 MBAdobe PDFView/Open

This item is protected by original copyright

Items in Eldorado are protected by copyright, with all rights reserved, unless otherwise indicated.