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dc.contributor.authorMolagoda, Ilandarage Menu Neelaka-
dc.contributor.authorLee, Seunghun-
dc.contributor.authorJayasooriya, Rajapaksha Gedara Prasad Tharanga-
dc.contributor.authorJin, Cheng-Yung-
dc.contributor.authorChoi, Yung Hyun-
dc.contributor.authorKim, Gi-Young-
dc.description.abstractDeoxynivalenol (DON) is one of the most common fungal toxins that contaminate food grains and cereal-derived products. However, it is unknown whether DON stimulates IL-1 β expression through the activation of the nuclear factor- κ B (NF- κ B) pathway and the ACS/NLRP3 inflammasome. In this study, we found that high concentrations of DON (above 800 nM) decreased relative cell viability; ho wever, no significant population of apoptotic sub-G 1 cells was observed. DON also upregulated IL-1 β expression from between 0.5 h and 6 h after treatment, and enhanced the nuclear localization of the NF- κ B subunits, p50 and p65. NF- κ B inhibitors, pyrrolidinedithiocarba- mate and PS1145, significantly suppressed the DON-induced IL-1 β expression, which indicated that DON in- creased IL-1 β expression through the activation of NF- κ B. In addition, marked secretion of IL-1 β protein occurred in the presence of DON at 24 h, and a caspase-1 inhibitor suppressed DON-mediated IL-1 β secretion, which sug- gested that caspase-1 indu ced the cleavage of pro-IL-1 β to lead the secretion of its active form. Thus, components of the inflammasome, such as ASC and NLRP3, significantly increased by DON treatment; in addition, the knock- down of ASC and NLRP3 markedly downregulated DON-induced IL-1 β secretion, but not IL-1 β gene expression, which indicated that DON promoted IL-1 β secretion through the ASC/NLRP3 inflammasome. Co llectively, the data suggested that DON induced IL-1 β expression in BV2 microglial cells through the activation of the NF- κ B signaling pathway and the subsequent upregulation of the ASC/NLRP3 inflammasome. Therefore, DON may in- duce inflammatory diseases or disorders by activating IL-1 β expression.en
dc.relation.ispartofseriesEXCLI Journal;Vol. 18 2019-
dc.subjectIL-1 βen
dc.subjectNuclear factor- κ Ben
dc.titleDeoxynivalenol enhances IL-1ß expression in BV2 microglial cells through activation of the NF-κB pathway and the ASC/NLRP3 inflammasomeen
dcterms.accessRightsopen access-
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