Authors: | Kaviani, Maryam Keshtkar, Somayeh Azarpira, Negar Hossein Aghdaei, Mahdokht Geramizadeh, Bita Karimi, Mohammad Hossein Yaghobi, Ramin Esfandiari, Elaheh Shamsaeefar, Alireza Nikeghbalian, Saman Al-Abdullah, Ismail H. |
Title: | Cytoprotective effects of ginsenoside Rd on apoptosis-associated cell death in the isolated human pancreatic islets |
Language (ISO): | en |
Abstract: | Ginsenoside Rd (GS-Rd), one of the main pharmacologically active components of ginseng, has shown the poten- tial to stabilize mitochondrial membrane integrity and decr ease apoptotic death in neuronal and non-neuronal cells. The present study aimed to evaluate the effect of this bi oactive molecule on the apopto sis-associated cell death in human pancreatic islets. In this regard human pancreatic islets were isolated and grouped for the treatment with GS-Rd. The isolated islets were treated with different concentrations of GS-Rd. After 24 and 72 h of incubation, the islets were evaluated in terms of viability, BAX , BCL2 , and insulin gene expression, BAX, BCL2, and caspase- 3 protein expression, apoptosis, and glucose-induced insu lin/C-peptide secretion. Our results revealed the islet survival was significantly decreased in the control group after 72 h of incubation. However, GS-Rd inhibited the progress of the islet death in the treated groups. TUNEL st aining revealed that the prev entive effect of this mole- cule was caused by the inhibition of apoptosis-associated death. In this regard, th e activation of caspase-3 was down-regulated in the presence of GS-Rd. GS-Rd did not exhibit undesirable effects on glucose-induced insulin and C-peptide stimulation secretion. In conclusion, GS-Rd inhibited the progress of death of cultured human pan- creatic islets by diminishing the apoptosis of the islet cells. |
Subject Headings: | Apoptosis Culture Ginsenoside Rd Human pancreatic islets Insulin Transplantation |
URI: | http://hdl.handle.net/2003/39045 http://dx.doi.org/10.17877/DE290R-20964 |
Issue Date: | 2019-08-22 |
Rights link: | http://creativecommons.org/licenses/by/4.0/ |
Appears in Collections: | Original Articles |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Azarpira_22082019_proof.pdf | DNB | 617.79 kB | Adobe PDF | View/Open |
This item is protected by original copyright |
This item is licensed under a Creative Commons License