Autor(en): Jarros, Isabele Carrilho
Veiga, Flávia Franco
Corrêa, Jakeline Luiz
Barros, Isabella Letícia Esteves
Gadelha, Marina Cristina
Voidaleski, Morgana F.
Pieralisi, Neli
Pedroso, Raissa Bocchi
Vicente, Vânia A.
Negri, Melyssa
Svidzinski, Terezinha Inez Estivalet
Titel: Microbiological and virulence aspects of Rhodotorula mucilaginosa
Sprache (ISO): en
Zusammenfassung: We aimed to characterize microbiologically clinical isolates of R. mucilaginosa isolated from colonization of a patient with chronic renal disease (CKD), as well as to evaluate their phylogeny, antifungal susceptibility, virulence, and pathogenicity in order to infer the potential to become a possible infective agent. For this study, two isolates of R. mucilaginosa from oral colonization of a CKD patient were isolated, identified and characterized by classical (genotypic and phenotypic) methods. Susceptibility to conventional antifungals was evaluated, followed by biofilm production, measured by different techniques (total biomass, metabolic activity, colony forming units and extracellular matrix quantification). Finally, the pathogenicity of yeast was evaluated by infection of Tenebrio molitor larvae. All isolates were resistant to azole and sensitive to polyenes and they were able to adhere and form biofilm on the abiotic surface of polystyrene. In general, similar profiles among isolates were observed over the observed periods (2, 24, 48 and 72 hours). Regarding extracellular matrix components of biofilms at different maturation ages, R. mucilaginosa was able to produce eDNA, eRNA, proteins, and polysaccharides that varied according to time and the strain. The death curve in vivo model showed a large reduction in the survival percentage of the larvae was observed in the first 24 hours, with only 40 % survival at the end of the evaluation. We infer that colonization of chronic renal patients by R. mucilaginosa offers a high risk of serious infection. And also emphasize that the correct identification of yeast is the main means for an efficient treatment.
Schlagwörter: Rhodotorula mucilaginosa
Characterization of biofilm
Tenebrio molitor
URI: http://hdl.handle.net/2003/39875
http://dx.doi.org/10.17877/DE290R-21766
Herausgeberlink: https://www.excli.de/index.php/excli/article/view/1672
Erscheinungsdatum: 2020-05-27
Rechte (Link): https://creativecommons.org/licenses/by/4.0/
Provinienz: IfADo - Leibniz Research Centre for Working Environment and Human Factors, Dortmund
Zitierform: Jarros, I. C., Veiga, F. F., Corrêa, J. L., Barros, I. L. E., Gadelha, M. C., Voidaleski, M. F., Pieralisi, N., Pedroso, R. B., Vicente, V. A., Negri, M., & Svidzinski, T. I. E. (2020). Microbiological and virulence aspects of Rhodotorula mucilaginosa. EXCLI Journal, 19, 687-704. https://doi.org/10.17179/excli2019-1672
Enthalten in den Sammlungen:Original Articles 2020

Dateien zu dieser Ressource:
Datei Beschreibung GrößeFormat 
excli2019-1672.pdfDNB1.37 MBAdobe PDFÖffnen/Anzeigen


Diese Ressource ist urheberrechtlich geschützt.



Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons Creative Commons