Cytoprotective effects of ginsenoside Rd on apoptosis-associated cell death in the isolated human pancreatic islets
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Date
2019-08-22
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Abstract
Ginsenoside Rd (GS-Rd), one of the main pharmacologically active components of ginseng, has shown the poten-
tial to stabilize mitochondrial membrane integrity and decr
ease apoptotic death in neuronal and non-neuronal cells.
The present study aimed to evaluate the effect of this bi
oactive molecule on the apopto
sis-associated cell death in
human pancreatic islets. In this regard human pancreatic islets were isolated and grouped for the treatment with
GS-Rd. The isolated islets were treated with different concentrations of GS-Rd. After 24 and 72 h of incubation,
the islets were evaluated in terms of viability,
BAX
,
BCL2
, and insulin gene expression, BAX, BCL2, and caspase-
3 protein expression, apoptosis, and glucose-induced insu
lin/C-peptide secretion. Our results revealed the islet
survival was significantly decreased in the control group after 72 h of incubation. However, GS-Rd inhibited the
progress of the islet death in the treated groups. TUNEL st
aining revealed that the prev
entive effect of this mole-
cule was caused by the inhibition of apoptosis-associated
death. In this regard, th
e activation of caspase-3 was
down-regulated in the presence of GS-Rd. GS-Rd did not exhibit undesirable effects on glucose-induced insulin
and C-peptide stimulation secretion. In conclusion, GS-Rd inhibited the progress of death of cultured human pan-
creatic islets by diminishing the apoptosis of the islet cells.
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Keywords
Apoptosis, Culture, Ginsenoside Rd, Human pancreatic islets, Insulin, Transplantation