Cytoprotective effects of ginsenoside Rd on apoptosis-associated cell death in the isolated human pancreatic islets
| dc.contributor.author | Kaviani, Maryam | |
| dc.contributor.author | Keshtkar, Somayeh | |
| dc.contributor.author | Azarpira, Negar | |
| dc.contributor.author | Hossein Aghdaei, Mahdokht | |
| dc.contributor.author | Geramizadeh, Bita | |
| dc.contributor.author | Karimi, Mohammad Hossein | |
| dc.contributor.author | Yaghobi, Ramin | |
| dc.contributor.author | Esfandiari, Elaheh | |
| dc.contributor.author | Shamsaeefar, Alireza | |
| dc.contributor.author | Nikeghbalian, Saman | |
| dc.contributor.author | Al-Abdullah, Ismail H. | |
| dc.date.accessioned | 2020-03-06T13:05:14Z | |
| dc.date.available | 2020-03-06T13:05:14Z | |
| dc.date.issued | 2019-08-22 | |
| dc.description.abstract | Ginsenoside Rd (GS-Rd), one of the main pharmacologically active components of ginseng, has shown the poten- tial to stabilize mitochondrial membrane integrity and decr ease apoptotic death in neuronal and non-neuronal cells. The present study aimed to evaluate the effect of this bi oactive molecule on the apopto sis-associated cell death in human pancreatic islets. In this regard human pancreatic islets were isolated and grouped for the treatment with GS-Rd. The isolated islets were treated with different concentrations of GS-Rd. After 24 and 72 h of incubation, the islets were evaluated in terms of viability, BAX , BCL2 , and insulin gene expression, BAX, BCL2, and caspase- 3 protein expression, apoptosis, and glucose-induced insu lin/C-peptide secretion. Our results revealed the islet survival was significantly decreased in the control group after 72 h of incubation. However, GS-Rd inhibited the progress of the islet death in the treated groups. TUNEL st aining revealed that the prev entive effect of this mole- cule was caused by the inhibition of apoptosis-associated death. In this regard, th e activation of caspase-3 was down-regulated in the presence of GS-Rd. GS-Rd did not exhibit undesirable effects on glucose-induced insulin and C-peptide stimulation secretion. In conclusion, GS-Rd inhibited the progress of death of cultured human pan- creatic islets by diminishing the apoptosis of the islet cells. | en |
| dc.identifier.issn | 1611-2156 | |
| dc.identifier.uri | http://hdl.handle.net/2003/39045 | |
| dc.identifier.uri | http://dx.doi.org/10.17877/DE290R-20964 | |
| dc.language.iso | en | |
| dc.relation.ispartofseries | EXCLI Journal;Vol. 18 2019 | |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
| dc.subject | Apoptosis | en |
| dc.subject | Culture | en |
| dc.subject | Ginsenoside Rd | en |
| dc.subject | Human pancreatic islets | en |
| dc.subject | Insulin | en |
| dc.subject | Transplantation | en |
| dc.subject.ddc | 610 | |
| dc.title | Cytoprotective effects of ginsenoside Rd on apoptosis-associated cell death in the isolated human pancreatic islets | en |
| dc.type | Text | |
| dc.type.publicationtype | article | |
| dcterms.accessRights | open access | |
| eldorado.dnb.zdberstkatid | 2132560-1 | |
| eldorado.secondarypublication | true |