Impaired immunomodulatory ability of type 2 diabetic adipose-derived mesenchymal stem cells in regulation of inflammatory condition in mixed leukocyte reaction
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Date
2019-09-23
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Abstract
The immunomodulatory properties of type 2 diabetic patients’ adipose-derived mesenchymal stem cells (D-ASCs)
has not been extensively studied. In this study, we compared the immunomodulatory properties of D-ASCs and
non-diabetic subjects mesenchymal stem cells (ND-ASCs)
in co-culture with mixed
leukocyte reaction (MLR).
ASCs were isolated from adipose tissue samples of type 2 diabetic and non-diabetic subjects (age: 40-55). D-ASCs
and ND-ASCs were co-cultured with two-way MLR. Peri
pheral blood mononuclear ce
lls (PBMCs) proliferation
ratio, protein levels of IFN-
γ
and IL-10, mRNA expression of
COX-2
,
TNF-
α
,
TGF-
β
1
and
IL-6
genes in MLR,
D-ASCs and ND-ASCs co-cultures were assessed using XTT, ELISA and Real-time qRT-PCR, respectively.
PBMCs proliferation on days 2 and 4 of D-ASCs co-culture was higher than ND-ASCs co-culture of the same
days (
p
< 0.001). IFN-
γ
level decreased on day 4 compared to day 2 of ND-ASCs co-culture, but its level had not
changed in D-ASCs co-culture.
COX-2
expression on days 2 and 4 of D-ASCs co-culture was lower than ND-
ASCs co-culture of the same days (
p
< 0.05). The expression of
TNF-
α
and
IL-6
on days 2 and 4 of D-ASCs co-
culture were higher than ND-ASCs co-culture of the same days (
p
< 0.001).
TGF-
β
1
on day 4 of ND-ASCs co-
culture showed a slightly higher expression than D-ASCs co-culture of the same day. Lower suppression of
PBMCs proliferation, declined expre
ssion of anti-inflammatory and upregulated expression of pro-inflammatory
factors in D-ASCs co-culture, indicated an impairment of
these cells in modulation of the inflammatory condition.
Description
Table of contents
Keywords
Type 2 diabetes, Immunomodulation, Mesenchymal stem cells, Adipose tissue, Inflammation