Impaired immunomodulatory ability of type 2 diabetic adipose-derived mesenchymal stem cells in regulation of inflammatory condition in mixed leukocyte reaction
| dc.contributor.author | Aliakbari, Sara | |
| dc.contributor.author | Mohammadi, Mobin | |
| dc.contributor.author | Rezaee, Mohammad Ali | |
| dc.contributor.author | Amini, Abbas Ali | |
| dc.contributor.author | Fakhari, Shohreh | |
| dc.contributor.author | Rahmani, Mohammad Reza | |
| dc.date.accessioned | 2020-03-06T14:15:56Z | |
| dc.date.available | 2020-03-06T14:15:56Z | |
| dc.date.issued | 2019-09-23 | |
| dc.description.abstract | The immunomodulatory properties of type 2 diabetic patients’ adipose-derived mesenchymal stem cells (D-ASCs) has not been extensively studied. In this study, we compared the immunomodulatory properties of D-ASCs and non-diabetic subjects mesenchymal stem cells (ND-ASCs) in co-culture with mixed leukocyte reaction (MLR). ASCs were isolated from adipose tissue samples of type 2 diabetic and non-diabetic subjects (age: 40-55). D-ASCs and ND-ASCs were co-cultured with two-way MLR. Peri pheral blood mononuclear ce lls (PBMCs) proliferation ratio, protein levels of IFN- γ and IL-10, mRNA expression of COX-2 , TNF- α , TGF- β 1 and IL-6 genes in MLR, D-ASCs and ND-ASCs co-cultures were assessed using XTT, ELISA and Real-time qRT-PCR, respectively. PBMCs proliferation on days 2 and 4 of D-ASCs co-culture was higher than ND-ASCs co-culture of the same days ( p < 0.001). IFN- γ level decreased on day 4 compared to day 2 of ND-ASCs co-culture, but its level had not changed in D-ASCs co-culture. COX-2 expression on days 2 and 4 of D-ASCs co-culture was lower than ND- ASCs co-culture of the same days ( p < 0.05). The expression of TNF- α and IL-6 on days 2 and 4 of D-ASCs co- culture were higher than ND-ASCs co-culture of the same days ( p < 0.001). TGF- β 1 on day 4 of ND-ASCs co- culture showed a slightly higher expression than D-ASCs co-culture of the same day. Lower suppression of PBMCs proliferation, declined expre ssion of anti-inflammatory and upregulated expression of pro-inflammatory factors in D-ASCs co-culture, indicated an impairment of these cells in modulation of the inflammatory condition. | en |
| dc.identifier.issn | 1611-2156 | |
| dc.identifier.uri | http://hdl.handle.net/2003/39053 | |
| dc.identifier.uri | http://dx.doi.org/10.17877/DE290R-20972 | |
| dc.language.iso | en | |
| dc.relation.ispartofseries | EXCLI Journal;Vol. 18 2019 | |
| dc.source.uri | http://creativecommons.org/licenses/by/4.0/ | |
| dc.subject | Type 2 diabetes | en |
| dc.subject | Immunomodulation | en |
| dc.subject | Mesenchymal stem cells | en |
| dc.subject | Adipose tissue | en |
| dc.subject | Inflammation | en |
| dc.subject.ddc | 610 | |
| dc.title | Impaired immunomodulatory ability of type 2 diabetic adipose-derived mesenchymal stem cells in regulation of inflammatory condition in mixed leukocyte reaction | en |
| dc.type | Text | |
| dc.type.publicationtype | article | |
| dcterms.accessRights | open access | |
| eldorado.dnb.zdberstkatid | 2132560-1 | |
| eldorado.secondarypublication | true |