Development of an automated flow chemistry affinity-based purification process for DNA-encoded chemistry

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dc.contributor.authorDinter, Robin
dc.contributor.authorGötte, Katharina
dc.contributor.authorGronke, Franziska
dc.contributor.authorJusten, Leon
dc.contributor.authorBrunschweiger, Andreas
dc.contributor.authorKockmann, Norbert
dc.date.accessioned2025-03-14T08:51:52Z
dc.date.available2025-03-14T08:51:52Z
dc.date.issued2023-09-26
dc.description.abstractAn automated flow chemistry platform for DNA-encoded library (DEL) technologies requires the integration of a purification process for DNA-tagged substrates. It facilitates the development of further DEL reactions, building block rehearsal, and library synthesis. Therefore, a recently developed, manual affinity-based batch purification process for DNA-tagged substrates based on dispersive solid-phase extraction (DSPE) was transferred to automated flow chemistry using tailored 3D-printed microfluidic devices and open-source lab automation equipment. The immobilization and purification steps use Watson–Crick base pairing for a compound-encoding single-stranded DNA, which allows for the thorough removal of impurities and contaminations by washing steps and operationally simple recovery of the purified DNA-encoded compounds. This work optimized the annealing step for flow incubation and DNA purification was accomplished by flow DSPE washing/elution steps. The manually performed batch affinity-based purification process was compared with the microfluidic process by determining qualitative and quantitative DNA recovery parameters. It aimed at comparing batch and flow purification processes with regard to DNA recovery and purity to benefit from the high potential for automation, precise process control, and higher information density of the microfluidic purification process for DNA-tagged substrates. Manual operations were minimized by applying an automation strategy to demonstrate the potential for integrating the microfluidic affinity-based purification process for DNA-tagged substrates into an automated DNA-encoded flow chemistry platform.en
dc.identifier.urihttp://hdl.handle.net/2003/43536
dc.identifier.urihttp://dx.doi.org/10.17877/DE290R-25369
dc.language.isoen
dc.relation.ispartofseriesJournal of flow chemistry; 13(4)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectDNA-encoded chemistryen
dc.subjectDNA purificationen
dc.subjectDispersive solid-phase extraction (DSPE)en
dc.subjectAffinity-based purificationen
dc.subjectMicrofluidicen
dc.subjectFlow chemistryen
dc.subject3D-printed lab equipmenten
dc.subjectOpen-source lab automationen
dc.subject.ddc660
dc.titleDevelopment of an automated flow chemistry affinity-based purification process for DNA-encoded chemistryen
dc.typeText
dc.type.publicationtypeArticle
dcterms.accessRightsopen access
eldorado.secondarypublicationtrue
eldorado.secondarypublication.primarycitationDinter, R. et al. (2023) ‘Development of an automated flow chemistry affinity-based purification process for DNA-encoded chemistry’, Journal of flow chemistry, 13(4), pp. 361–373. Available at: https://doi.org/10.1007/s41981-023-00282-0
eldorado.secondarypublication.primaryidentifierhttps://doi.org/10.1007/s41981-023-00282-0

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